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Clin Chim Acta. 2018 Jan;476:15-24. doi: 10.1016/j.cca.2017.10.030. Epub 2017 Nov 6.

The Simultaneous measurement of serum testosterone and 5α-dihydrotestosterone by gas chromatography-mass spectrometry (GC-MS).

Author information

1
Center for Laboratory Medicine, University Hospital Münster, Münster, Germany.
2
Institute for Transfusion Medicine and Transplantation Immunology, University Hospital Münster, Münster, Germany.
3
Center for Reproductive Medicine and Andrology, Department of Clinical Andrology, University Hospital Münster, Münster, Germany.
4
Center for Laboratory Medicine, University Hospital Münster, Münster, Germany. Electronic address: nofer@uni-muenster.de.
5
UKM Kinderwunschzentrum, Department of Gynaecology and Obstetrics, University Hospital Münster, Münster, Germany.

Abstract

BACKGROUND:

Simultaneous measurement of testosterone (T) and 5α-dihydrotestosterone (DHT) is important for diagnosing androgen deficiency states and hyperandrogenism in males and females, respectively. However, immunoassays used for T and DHT determination suffer from inadequate specificity and sensitivity, while tandem mass spectrometry is expensive and demanding in use.

METHODS AND RESULTS:

We developed a selective gas chromatography-mass spectrometry (GC-MS) method for parallel T and DHT measurement. The assay showed a linear response up to 46.5nmol/L, intra- and interassay imprecision and inaccuracy <15% and recoveries in spiked samples >90% for both analytes. The limit of quantitation was 0.117nmol/L for T and 0.168nmol/L for DHT. Comparison with immunoassays revealed good agreement for T in males, but a bias in favour of immunoassays at low concentrations for T in females and DHT in both sexes. We established reference ranges for T and DHT and suggest interval partitioning for T according to age in men and menstrual cycle in women. Assay validation in a clinical setting suggests that measuring DHT or T/DHT ratio may help identify patients with polycystic ovary syndrome.

CONCLUSION:

We developed a selective, simple and inexpensive GC-MS method for parallel measurement of T and DHT with potential use in the clinical laboratory.

KEYWORDS:

Dihydrotestosterone; Gas chromatography–mass spectrometry; Polycystic ovary syndrome (PCOS); Reference intervals; Testosterone

PMID:
29122541
DOI:
10.1016/j.cca.2017.10.030
[Indexed for MEDLINE]

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