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ACS Nano. 2017 Dec 26;11(12):11847-11855. doi: 10.1021/acsnano.7b04992. Epub 2017 Nov 13.

Nanodisc-Based Bioelectronic Nose Using Olfactory Receptor Produced in Escherichia coli for the Assessment of the Death-Associated Odor Cadaverine.

Author information

1
School of Chemical and Biological Engineering, Seoul National University , Seoul 08826, Republic of Korea.
2
Department of Biophysics and Chemical Biology, Seoul National University , Seoul 08826, Republic of Korea.
3
Department of Physics and Astronomy and Institute of Applied Physics, Seoul National University , Seoul 08826, Republic of Korea.
4
Division of Bioconvergence Analysis, Korea Basic Science Institute (KBSI) , Daejeon 34133, Republic of Korea.
5
Center for Convergent Research of Emerging Virus Infection, Korea Research Institute of Chemical Technology , Daejeon 34114, Republic of Korea.

Abstract

Cadaverine (CV), a death-associated odor, is an important target molecule for various sensor applications, including the evaluation of food spoilage. In this study, we developed an oriented nanodisc (ND)-functionalized bioelectronic nose (ONBN), based on carbon nanotube transistors and nanodiscs embedded with an olfactory receptor produced in Escherichia coli (E. coli) for detection of CV. To fabricate ONBN devices, a trace-amine-associated receptor 13c (TAAR13c) binding to CV was produced in E. coli, purified, reconstituted into NDs, and assembled, in the desired orientation, onto a carbon- nanotube-based field-effect transistor with floating electrodes. The ONBN showed high performance in terms of sensitivity and selectivity. Moreover, the ONBN was used to measure CV in diverse real-food samples for the determination of food freshness. These results indicate ONBN devices can be utilized to evaluate the quality of food samples quantitatively, which should enable versatile practical applications such as food safety and preservative development. Moreover, the ONBN could provide a useful tool for detection of corpses, which could be practically used in disaster responses.

KEYWORDS:

bioelectronic nose; cadaverine; field-effect transistor; nanodiscs; trace-amine-associated receptor

PMID:
29121467
DOI:
10.1021/acsnano.7b04992
[Indexed for MEDLINE]

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