Format

Send to

Choose Destination
ACS Chem Biol. 2018 Feb 16;13(2):347-356. doi: 10.1021/acschembio.7b00800. Epub 2017 Dec 5.

Diverse Class 2 CRISPR-Cas Effector Proteins for Genome Engineering Applications.

Pyzocha NK1,2, Chen S3,4,5,6,7,8.

Author information

1
Broad Institute of MIT and Harvard , Cambridge, Massachusetts 02142, United States.
2
Department of Biology, Massachusetts Institute of Technology , Cambridge, Massachusetts 02139, United States.
3
Department of Genetics, Yale University , 333 Cedar Street, New Haven, Connecticut 06510, United States.
4
System Biology Institute , 850 West Campus Drive, ISTC 361, West Haven, Connecticut 06516, United States.
5
MCGD Program, Yale University , 333 Cedar Street, New Haven, Connecticut 06510, United States.
6
Immunobiology Program, Yale University , 300 Cedar Street, New Haven, Connecticut 06520, United States.
7
Comprehensive Cancer Center, Yale University , New Haven, Connecticut 06510, United States.
8
Stem Cell Center, Yale University , New Haven, Connecticut 06510, United States.

Abstract

CRISPR-Cas genome editing technologies have revolutionized modern molecular biology by making targeted DNA edits simple and scalable. These technologies are developed by domesticating naturally occurring microbial adaptive immune systems that display wide diversity of functionality for targeted nucleic acid cleavage. Several CRISPR-Cas single effector enzymes have been characterized and engineered for use in mammalian cells. The unique properties of the single effector enzymes can make a critical difference in experimental use or targeting specificity. This review describes known single effector enzymes and discusses their use in genome engineering applications.

PMID:
29121460
DOI:
10.1021/acschembio.7b00800
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for American Chemical Society
Loading ...
Support Center