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Curr Protoc Microbiol. 2017 Nov 9;47:3E.1.1-3E.1.10. doi: 10.1002/cpmc.40.

Azospirillum brasilense, a Beneficial Soil Bacterium: Isolation and Cultivation.

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Biochemistry & Cellular and Molecular Biology, The University of Tennessee, Knoxville, Tennessee.


Bacteria of the genus Azospirillum comprise 15 species to date, with A. brasilense the best studied species in the genus. Azospirillum are soil bacteria able to promote the growth of plants from 113 species spanning 35 botanical families. These non-pathogenic and beneficial bacteria are ubiquitous in soils and inhabit the roots of diverse plants. These bacteria are microaerophilic, able to fix nitrogen under free-living conditions, motile, and able to navigate in gradients of various chemicals, including oxygen. These physiological traits are used to isolate these soil bacteria from soil and plant root samples, providing isolates that can be used for studying microbial physiology and plant growth promotion.


Azospirillum; chemotaxis; motility; nitrogen-fixation; plant roots

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