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Cell Rep. 2017 Nov 7;21(6):1461-1470. doi: 10.1016/j.celrep.2017.10.047.

Drosophila Embryonic Hemocytes Produce Laminins to Strengthen Migratory Response.

Author information

1
CABD (CSIC-Universidad Pablo de Olavide-JA), Sevilla 41013, Spain; Randall Centre for Cell and Molecular Biophysics, King's College London, London SE5 9AP, UK.
2
Department of Physiology, Development and Neuroscience, University of Cambridge, Cambridge CB2 3DY, UK.
3
Department of Cellular and Molecular Medicine, Biomedical Sciences, University of Bristol, Bristol BS8 1TD, UK.
4
Randall Centre for Cell and Molecular Biophysics, King's College London, London SE5 9AP, UK.
5
CABD (CSIC-Universidad Pablo de Olavide-JA), Sevilla 41013, Spain. Electronic address: mdmarber@upo.es.

Abstract

The most prominent developmental function attributed to the extracellular matrix (ECM) is cell migration. While cells in culture can produce ECM to migrate, the role of ECM in regulating developmental cell migration is classically viewed as an exogenous matrix presented to the moving cells. In contrast to this view, we show here that Drosophila embryonic hemocytes deposit their own laminins in streak-like structures to migrate efficiently throughout the embryo. With the help of transplantation experiments, live microscopy, and image quantification, we demonstrate that autocrine-produced laminin regulates hemocyte migration by controlling lamellipodia dynamics, stability, and persistence. Proper laminin deposition is regulated by the RabGTPase Rab8, which is highly expressed and required in hemocytes for lamellipodia dynamics and migration. Our results thus support a model in which, during embryogenesis, the Rab8-regulated autocrine deposition of laminin reinforces directional and effective migration by stabilizing cellular protrusions and strengthening otherwise transient adhesion states.

KEYWORDS:

Drosophila; cell migration; extracellular matrix; hemocytes; lamellipodia dynamics; laminins

PMID:
29117553
PMCID:
PMC5695906
DOI:
10.1016/j.celrep.2017.10.047
[Indexed for MEDLINE]
Free PMC Article

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