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Proc Natl Acad Sci U S A. 2017 Nov 21;114(47):E10112-E10121. doi: 10.1073/pnas.1708367114. Epub 2017 Nov 7.

Structure and function of yeast Atg20, a sorting nexin that facilitates autophagy induction.

Author information

1
Life Sciences Institute, University of Michigan, Ann Arbor, MI 48109.
2
Department of Chemistry, Dartmouth College, Hanover, NH 03755.
3
Laboratory of Cell and Molecular Biology, National Institutes of Health, Bethesda, MD 20892.
4
Life Sciences Institute, University of Michigan, Ann Arbor, MI 48109; klionsky@umich.edu michael.j.ragusa@dartmouth.edu.
5
Department of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor, MI 48109.
6
Department of Chemistry, Dartmouth College, Hanover, NH 03755; klionsky@umich.edu michael.j.ragusa@dartmouth.edu.

Abstract

The Atg20 and Snx4/Atg24 proteins have been identified in a screen for mutants defective in a type of selective macroautophagy/autophagy. Both proteins are connected to the Atg1 kinase complex, which is involved in autophagy initiation, and bind phosphatidylinositol-3-phosphate. Atg20 and Snx4 contain putative BAR domains, suggesting a possible role in membrane deformation, but they have been relatively uncharacterized. Here we demonstrate that, in addition to its function in selective autophagy, Atg20 plays a critical role in the efficient induction of nonselective autophagy. Atg20 is a dynamic posttranslationally modified protein that engages both structurally stable (PX and BAR) and intrinsically disordered domains for its function. In addition to its PX and BAR domains, Atg20 uses a third membrane-binding module, a membrane-inducible amphipathic helix present in a previously undescribed location in Atg20 within the putative BAR domain. Taken together, these findings yield insights into the molecular mechanism of the autophagy machinery.

KEYWORDS:

autophagy; vacuole; yeast

PMID:
29114050
PMCID:
PMC5703286
DOI:
10.1073/pnas.1708367114
[Indexed for MEDLINE]
Free PMC Article

Conflict of interest statement

The authors declare no conflict of interest.

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