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J Agric Food Chem. 2017 Dec 6;65(48):10482-10488. doi: 10.1021/acs.jafc.7b04522. Epub 2017 Nov 17.

New ACE-Inhibitory Peptides from Hemp Seed (Cannabis sativa L.) Proteins.

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Department of Chemistry, University of Milan , via C. Golgi 19, I-20133 Milano, Italy.
Department of Pharmaceutical Sciences, University of Milan , via L. Mangiagalli 25, I-20133 Milan, Italy.
Institute for Macromolecular Studies (ISMAC-CNR) , via A. Corti 12, I-20133 Milan, Italy.
Institute of Molecular Science and Technologies (ISTM-CNR) , via C. Golgi 19, I-20133 Milan, Italy.


A hemp seed protein isolate, prepared from defatted hemp seed meals by alkaline solubilization/acid precipitation, was subjected to extensive chemical hydrolysis under acid conditions (6 M HCl). The resulting hydrolysate was fractionated by semipreparative RP-HPLC, and the purified fractions were tested as inhibitors of angiotensin converting enzyme (ACE). Mono- and bidimensional NMR experiments and LC-MS analyses led to the identification of four potentially bioactive peptides, i.e. GVLY, IEE, LGV, and RVR. They were prepared by solid-phase synthesis, and tested for ACE-inhibitory activity. The IC50 values were GVLY 16 ± 1.5 μM, LGV 145 ± 13 μM, and RVR 526 ± 33 μM, confirming that hemp seed may be a valuable source of hypotensive peptides.


ACE-inhibitors; Cannabis sativa L.; LC-MS/MS; bioactive peptides; hemp seed protein hydrolysate

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