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Gene. 2018 Feb 5;642:212-219. doi: 10.1016/j.gene.2017.10.080. Epub 2017 Oct 28.

Recombinant latcripin 11 of Lentinula edodes C91-3 suppresses the proliferation of various cancer cells.

Author information

1
Department of Microbiology, Dalian Medical University, Dalian, Liaoning, China; Department of Biology, Shenyang Normal University, Shenyang, Liaoning, China.
2
Department of Microbiology, Dalian Medical University, Dalian, Liaoning, China.
3
Department of Critical Care Medicine, The First Affiliated Hospital of Dalian Medical University, Dalian, Liaoning, China.
4
Department of Hematology, The Second Affiliated Hospital of Dalian Medical University, Dalian, Liaoning, China.
5
Department of Medical Microbiology, Dalian Medical University, 9 Western Section, Lvshun South Road, Lvshunkou District, Dalian, Liaoning, China. Electronic address: huangminchao@163.com.

Abstract

Lentinula edodes C91-3 is an edible mushroom that has demonstrated a remarkable anti-tumor effect in various cancer cells both in vitro and in vivo. In the present study, we report the ability of recombinant thioredoxin-like latcripin 11 (LP-11) of Lentinula edodes C91-3 to suppress the proliferation of various cancer cells. The LP-11 gene of Lentinula edodes C91-3 was cloned in the pET-32a(+) expression vector and expressed in a prokaryotic system. The expressed protein was refolded by gradual dialysis and purified by affinity gel filtration chromatography. The antioxidant activity of LP-11 was tested by 1,1-dipheny l-2-picrylhydrazyl (DPPH) assay. The anti-tumor activity of recombinant LP-11 was tested in eight kinds of tumor cell lines by CCK-8 assay. Recombinant LP-11 significantly suppressed the proliferation of various cancer cells, but not normal human umbilical vein endothelial cells. Human lymphoma U937 cells exhibited the most sensitivity to LP-11 protein. U937 cell apoptosis was assessed by Annexin V staining coupled with flow cytometry, and mitochondrial morphology was analyzed by light and electron microscopy. It was revealed that recombinant LP-11 induced apoptosis in human leukemic monocyte lymphoma U937 cells. Our findings suggest that recombinant LP-11 is a promising agent for the treatment of lymphoma.

KEYWORDS:

Antioxidant; Antitumor; Expression; Latcripin-11; Lentinula edodes C91-3

PMID:
29111208
DOI:
10.1016/j.gene.2017.10.080
[Indexed for MEDLINE]

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