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J Physiol. 2018 Jan 15;596(2):145-162. doi: 10.1113/JP273463. Epub 2017 Dec 4.

Studies of cortical connectivity using optical circuit mapping methods.

Author information

1
New York University, New York, USA.
2
University College London, London, UK.
3
University of Oxford, Oxford, UK.

Abstract

An important consideration when probing the function of any neuron is to uncover the source of synaptic input onto the cell, its intrinsic physiology and efferent targets. Over the years, electrophysiological approaches have generated considerable insight into these properties in a variety of cortical neuronal subtypes and circuits. However, as researchers explore neuronal function in greater detail, they are increasingly turning to optical techniques to bridge the gap between local network interactions and behaviour. The application of optical methods has increased dramatically over the past decade, spurred on by the optogenetic revolution. In this review, we provide an account of recent innovations, providing researchers with a primer detailing circuit mapping strategies in the cerebral cortex. We will focus on technical aspects of performing neurotransmitter uncaging and channelrhodopsin-assisted circuit mapping, with the aim of identifying common pitfalls that can negatively influence the collection of reliable data.

KEYWORDS:

cerebral cortex; glutamate uncaging; neural circuits; optogenetics

PMID:
29110301
PMCID:
PMC5767689
DOI:
10.1113/JP273463
[Indexed for MEDLINE]
Free PMC Article

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