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Molecules. 2017 Oct 29;22(11). pii: E1823. doi: 10.3390/molecules22111823.

Asperflavin, an Anti-Inflammatory Compound Produced by a Marine-Derived Fungus, Eurotium amstelodami.

Author information

1
College of Chemical and Pharmaceutical Engineering, Jilin Institute of Chemical Technology, Jilin 132022, China. yangwt_1981@163.com.
2
Department of Marine Life Science, Jeju National University, Jeju 690-756, Korea. yangwt_1981@163.com.
3
Department of Marine Life Science, Jeju National University, Jeju 690-756, Korea. networksun@naver.com.
4
College of Pharmacy Science, Changchun University of Traditional Chinese Medicine, Changchun 130017, China. donaikun@163.com.
5
Department of Marine Life Science, Jeju National University, Jeju 690-756, Korea. yellow6798@hanmail.net.
6
Pediatric Oncology Experimental Therapeutics Investigators Consortium (POETIC) Laboratory for Pre-Clinical and Drug Discovery Studies, University of Calgary, Calgary, AB T2N1N4, Canada. tjdaud81@gmail.com.
7
Department of Marine Life Science, Jeju National University, Jeju 690-756, Korea. youjin2014@gmail.com.
8
Marine and Environmental Research Institute, Jeju National University, Jeju 695-814, Korea. youjin2014@gmail.com.

Abstract

In the present study, 16 marine-derived fungi were isolated from four types of marine materials including float, algae, animals and drift woods along with the coast of Jeju Island, Korea and evaluated for anti-inflammatory effects in lipopolysaccharide (LPS)-stimulated RAW 24.7 cells. The broth and mycelium extracts from the 16 fungi were prepared and the broth extract (BE) of Eurotium amstelodami (015-2) inhibited nitric oxide (NO) production in LPS-stimulated RAW 264.7 cells without cytotoxicity. By further bioassay-guided isolation, three compounds including asperflavin, neoechinulin A and preechinulin were successfully isolated from the BE of E. amstelodami. It was revealed that asperflavin showed no cytotoxicity up to 200 μM and significantly inhibited LPS-induced NO and PGE2 production in a dose-dependent manner. In the western blot results, asperflavin suppressed only inducible NOS (iNOS), but COX-2 were slightly down-regulated. Asperflavin was also observed to inhibit the production of pro-inflammatory cytokines including TNF-α, IL-1β, and IL-6. In conclusion, this study reports a potential use of asperflavin isolated from a marine fungus, E. amstelodami as an anti-inflammatory agent via suppression of iNOS and pro-inflammatory cytokines as well as no cytotoxicity.

KEYWORDS:

Anti-inflammation; Eurotium amstelodami; NO production; asperflavin; marine fungus

PMID:
29109367
PMCID:
PMC6150366
DOI:
10.3390/molecules22111823
[Indexed for MEDLINE]
Free PMC Article

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