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Clin Sci (Lond). 2017 Dec 8;131(24):2933-2945. doi: 10.1042/CS20171258. Print 2017 Dec 15.

The shifted balance between circulating follicular regulatory T cells and follicular helper T cells in patients with ulcerative colitis.

Author information

1
Department of Central Laboratory, First Hospital, Jilin University, Changchun 130021, China.
2
Department of Radiation Oncology, China-Japan Union Hospital of Jilin University, Changchun 130021, China.
3
Department of Nephrology, First Hospital, Jilin University, Changchun 130021, China.
4
Department of Central Laboratory, First Hospital, Jilin University, Changchun 130021, China pujungao@yeah.net yanfangjiang@hotmail.com.
5
Key Laboratory of Zoonosis Research, Ministry of Education, First Hospital, Jilin University, Changchun 130021, China.
6
Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, China.

Abstract

B-cell immunity participates in the pathogenesis of ulcerative colitis (UC). The immune balance between follicular regulatory T (TFR) cells and follicular helper T (TFH) cells is important in regulating B-cell responses. However, the alteration of TFR/TFH balance in UC remains unclear. Peripheral blood from 25 UC patients and 15 healthy controls was examined for the frequencies of circulating TFR, TFH, and regulatory T (Treg) cells by flow cytometry. Levels of serum cytokines were measured using cytometric bead array (CBA). Disease activity was evaluated by the Mayo Clinic Score. Compared with controls, UC patients exhibited significant reductions in circulating Foxp3+CXCR5+ TFR cells, the subset interleukin (IL)-10+Foxp3+CXCR5+ cells, and Treg cells, but significant expansions in Foxp3-CXCR5+ TFH cells and IL-21+Foxp3-CXCR5+ cells. UC patients also had reduced levels of serum IL-10 and elevated levels of serum IL-21. The values of Mayo Clinic Score, C-reactive protein (CRP), or erythrocyte sedimentation rate (ESR) in UC patients were negatively correlated with circulating TFR cells, serum IL-10 level, and TFR/TFH ratio, while positively correlated with circulating TFH cells and serum IL-21 level. Alterations in circulating TFR and TFH cells shift the balance from immune tolerance to immune responsive state, contributing to dysregulated B-cell immunity and the pathogenesis of UC.

KEYWORDS:

IL-10; IL-21; TFH cells; TFR cells; Ulcerative colitis

PMID:
29109300
DOI:
10.1042/CS20171258
[Indexed for MEDLINE]

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