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Fish Shellfish Immunol. 2018 Jan;72:210-219. doi: 10.1016/j.fsi.2017.10.062. Epub 2017 Nov 3.

Involvement of clip-domain serine protease in the anti-Vibrio immune response of abalone (Haliotis discus hannai)-Molecular cloning, characterization and functional analysis.

Author information

1
College of Food and Biological Engineering, Jimei University, Xiamen, Fujian Province 361100, China.
2
CAS Key Laboratory of Innate Immunity and Chronic Disease, CAS Center for Excellence in Molecular Cell Science, School of Life Sciences and Medical Center, University of Science & Technology of China, Hefei, Anhui Province 230007, China.
3
College of Food and Biological Engineering, Jimei University, Xiamen, Fujian Province 361100, China; Fujian Collaborative Innovation Center for Exploitation and Utilization of Marine Biological Resources, Xiamen, Fujian Province 361100, China.
4
College of Food and Biological Engineering, Jimei University, Xiamen, Fujian Province 361100, China; Fujian Collaborative Innovation Center for Exploitation and Utilization of Marine Biological Resources, Xiamen, Fujian Province 361100, China. Electronic address: mjcao@jmu.edu.cn.

Abstract

Vibrio parahemolyticus (V. parahemolyticus) is a major pathogen for abalone, an important economical shellfish in coastal area of China. There is little known about the abalone innate immune system against pathogen infection. Clip-domain serine proteases (cSPs) are increasingly recognized to play important roles in host immune defense in invertebrates. In this study, we cloned a cSP (Hdh-cSP) from abalone (Haliotis discus hannai). We found out that Hdh-cSP was widely expressed in multiple tissues of abalone, with highest level in the immune-like organ, hepatopancreas. V. parahemolyticus infection induced significantly elevated expression of Hdh-cSP in addition to better-characterized innate immune component genes including Rel/NF-κB, allograft inflammatory factor (ALInFa), macrophage expressed protein (MEP) and caspase-8. Importantly, the silencing of Hdh-cSP reduced the expression of these genes, suggesting that Hdh-cSP was an upstream regulatory factor in V. parahemolyticus infection. Further analysis showed that apoptosis of hemocytes was inhibited when the transcription of Hdh-cSP was knocked down, suggesting that Hdh-cSP participated in cell apoptosis by regulation of caspase 8 expression in V. parahemolyticus infection. Therefore, our study established an important role of cSP in the innate immunity against V. parahemolyticus infection in abalone.

KEYWORDS:

Apoptosis; Clip-domain serine protease; Haliotis discus hannai; Innate immunity; Vibrio parahemolyticus

PMID:
29108972
DOI:
10.1016/j.fsi.2017.10.062
[Indexed for MEDLINE]

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