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Dev Biol. 2018 Jan 1;433(1):17-32. doi: 10.1016/j.ydbio.2017.10.021. Epub 2017 Nov 3.

Retinoic acid temporally orchestrates colonization of the gut by vagal neural crest cells.

Author information

1
Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA 91125, USA; Department of Biosciences, Rice University, Houston, TX 77005, USA. Electronic address: rosa.uribe@rice.edu.
2
Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA 91125, USA.

Abstract

The enteric nervous system arises from neural crest cells that migrate as chains into and along the primitive gut, subsequently differentiating into enteric neurons and glia. Little is known about the mechanisms governing neural crest migration en route to and along the gut in vivo. Here, we report that Retinoic Acid (RA) temporally controls zebrafish enteric neural crest cell chain migration. In vivo imaging reveals that RA loss severely compromises the integrity and migration of the chain of neural crest cells during the window of time window when they are moving along the foregut. After loss of RA, enteric progenitors accumulate in the foregut and differentiate into enteric neurons, but subsequently undergo apoptosis resulting in a striking neuronal deficit. Moreover, ectopic expression of the transcription factor meis3 and/or the receptor ret, partially rescues enteric neuron colonization after RA attenuation. Collectively, our findings suggest that retinoic acid plays a critical temporal role in promoting enteric neural crest chain migration and neuronal survival upstream of Meis3 and RET in vivo.

KEYWORDS:

Enteric nervous system; Meis3; Neural crest; Retinoic Acid; Zebrafish

PMID:
29108781
PMCID:
PMC5722660
DOI:
10.1016/j.ydbio.2017.10.021
[Indexed for MEDLINE]
Free PMC Article

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