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Cytotherapy. 2017 Dec;19(12):1474-1490. doi: 10.1016/j.jcyt.2017.08.022. Epub 2017 Nov 6.

Comparative restoration of acute liver failure by menstrual blood stem cells compared with bone marrow stem cells in mice model.

Author information

1
Department of Molecular Medicine, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran.
2
Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran.
3
Department of Pathology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
4
The Ritchie Centre, Hudson Institute of Medical Research, and Department of Obstetrics and Gynaecology, Monash University, Melbourne, Victoria, Australia.
5
Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran. Electronic address: kazemnejad_s@yahoo.com.

Abstract

BACKGROUND AIMS:

The application of menstrual blood stem cells (MenSCs) in regenerative medicine is gaining increasing attention. The aim of this study was to investigate the therapeutic potential of MenSCs compared with bone marrow-derived stem cells (BMSCs) in an animal model of CCl4-induced acute hepatic failure.

METHODS:

Injured Balb/C mice were divided into multiple groups and received MenSCs, BMSCs or hepatocyte progenitor-like (HPL) cells derived from these cells.

RESULTS:

Tracking of green fluorescent protein-labeled cells showed homing of cells in injured areas of the liver. In addition, the liver engraftment of MenSCs was shown by immunofluorescence staining using anti-human mitochondrial antibody. Microscopically examination, periodic acid-Schiff and Masson's trichrome staining of liver sections demonstrated the considerable liver regeneration post-cell therapy in all groups. Assessment of serum parameters including aspartate aminotransferase, alanine aminotransferase, total bilirubin, urea and cholesterol at day 7 exhibited significant reduction, such that this downward trend continued significantly until day 30. The restoration of liver biochemical markers, changes in mRNA levels of hepatic markers and the suppression of inflammatory markers were more significant in the MenSC-treated group compared with the BMSC-treated group. On the other hand, HPL cells in reference to undifferentiated cells had better effectiveness in the treatment of the acute liver injury.

CONCLUSIONS:

Our data show that MenSCs may be considered an appropriate alternative stem cell population to BMSCs for treatment of acute liver failure.

KEYWORDS:

bone marrow stem cells; liver injury; menstrual blood stem cells; regenerative medicine

PMID:
29107739
DOI:
10.1016/j.jcyt.2017.08.022
[Indexed for MEDLINE]

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