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Stem Cell Reports. 2017 Nov 14;9(5):1463-1476. doi: 10.1016/j.stemcr.2017.09.023. Epub 2017 Oct 26.

Default Patterning Produces Pan-cortical Glutamatergic and CGE/LGE-like GABAergic Neurons from Human Pluripotent Stem Cells.

Author information

1
Department of Neurosciences, University of New Mexico-Health Science Center, Albuquerque, NM 87131, USA.
2
UNM Comprehensive Cancer Center, University of New Mexico-Health Science Center, Albuquerque, NM 87131, USA.
3
UNM Comprehensive Cancer Center, University of New Mexico-Health Science Center, Albuquerque, NM 87131, USA; Department of Internal Medicine, University of New Mexico-Health Science Center, Albuquerque, NM 87131, USA.
4
Department of Genetics, University of North Carolina School of Medicine, Chapel Hill, NC 27599, USA; UNC Neuroscience Center, University of North Carolina School of Medicine, Chapel Hill, NC 27599, USA.
5
Department of Neurosciences, University of New Mexico-Health Science Center, Albuquerque, NM 87131, USA. Electronic address: jpweick@salud.unm.edu.

Abstract

Default differentiation of human pluripotent stem cells has been promoted as a model of cortical development. In this study, a developmental transcriptome analysis of default-differentiated hPSNs revealed a gene expression program resembling in vivo CGE/LGE subpallial domains and GABAergic signaling. A combination of bioinformatic, functional, and immunocytochemical analysis further revealed that hPSNs consist of both cortical glutamatergic and CGE-like GABAergic neurons. This study provides a comprehensive characterization of the heterogeneous group of neurons produced by default differentiation and insight into future directed differentiation strategies.

KEYWORDS:

CALB2; CGE; COUPTFII; cortical neurons; development; human pluripotent stem cells; microarray; subpallium

PMID:
29107596
PMCID:
PMC5831028
DOI:
10.1016/j.stemcr.2017.09.023
[Indexed for MEDLINE]
Free PMC Article

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