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Stem Cell Reports. 2017 Nov 14;9(5):1706-1720. doi: 10.1016/j.stemcr.2017.09.013. Epub 2017 Oct 26.

Naked Mole Rat Induced Pluripotent Stem Cells and Their Contribution to Interspecific Chimera.

Author information

1
Division of Genetics, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA.
2
Division of Genetics, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA; Center for Data-Intensive Biomedicine and Biotechnology, Skolkovo Institute of Science and Technology, Moscow 143028, Russia.
3
Department of Agricultural Biotechnology, and Research Institute of Agriculture and Life Science, Seoul National University, Seoul 151-921, Korea.
4
Rodent Histopathology Laboratory, Harvard Medical School, Boston MA 02115, USA.
5
Department of Agricultural Biotechnology, and Research Institute of Agriculture and Life Science, Seoul National University, Seoul 151-921, Korea; Institute of Green Bio Science and Technology, Seoul National University, Pyeongchang, Gangwon-do 232-916, Korea.
6
Department of Biological Sciences, University of Illinois at Chicago, Chicago, IL 60607, USA.
7
Division of Genetics, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA. Electronic address: vgladyshev@rics.bwh.harvard.edu.

Abstract

Naked mole rats (NMRs) are exceptionally long-lived, cancer-resistant rodents. Identifying the defining characteristics of these traits may shed light on aging and cancer mechanisms. Here, we report the generation of induced pluripotent stem cells (iPSCs) from NMR fibroblasts and their contribution to mouse-NMR chimeric embryos. Efficient reprogramming could be observed under N2B27+2i conditions. The iPSCs displayed a characteristic morphology, expressed pluripotent markers, formed embryoid bodies, and showed typical differentiation patterns. Interestingly, NMR embryonic fibroblasts and the derived iPSCs had propensity for a tetraploid karyotype and were resistant to forming teratomas, but within mouse blastocysts they contributed to both interspecific placenta and fetus. Gene expression patterns of NMR iPSCs were more similar to those of human than mouse iPSCs. Overall, we uncovered unique features of NMR iPSCs and report a mouse-NMR chimeric model. The iPSCs and associated cell culture systems can be used for a variety of biological and biomedical applications.

KEYWORDS:

aging; induced pluripotent stem cells; interspecific chimera; lifespan; naked mole rat; reprogramming

PMID:
29107591
PMCID:
PMC5829328
DOI:
10.1016/j.stemcr.2017.09.013
[Indexed for MEDLINE]
Free PMC Article

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