Format

Send to

Choose Destination
Neuron. 2017 Nov 15;96(4):783-795.e4. doi: 10.1016/j.neuron.2017.10.011. Epub 2017 Oct 26.

Transsynaptic Mapping of Second-Order Taste Neurons in Flies by trans-Tango.

Author information

1
Department of Neuroscience, Division of Biology and Medicine, Brown University, Providence, RI 02912, USA.
2
Department of Neuroscience, Division of Biology and Medicine, Brown University, Providence, RI 02912, USA. Electronic address: gilad_barnea@brown.edu.

Abstract

Mapping neural circuits across defined synapses is essential for understanding brain function. Here we describe trans-Tango, a technique for anterograde transsynaptic circuit tracing and manipulation. At the core of trans-Tango is a synthetic signaling pathway that is introduced into all neurons in the animal. This pathway converts receptor activation at the cell surface into reporter expression through site-specific proteolysis. Specific labeling is achieved by presenting a tethered ligand at the synapses of genetically defined neurons, thereby activating the pathway in their postsynaptic partners and providing genetic access to these neurons. We first validated trans-Tango in the Drosophila olfactory system and then implemented it in the gustatory system, where projections beyond the first-order receptor neurons are not fully characterized. We identified putative second-order neurons within the sweet circuit that include projection neurons targeting known neuromodulation centers in the brain. These experiments establish trans-Tango as a flexible platform for transsynaptic circuit analysis.

KEYWORDS:

anterograde; anterograde tracing; gustatory system; neural circuit; olfactory system; postsynaptic; second-order neuron; trans-Tango; transsynaptic tracing

PMID:
29107518
PMCID:
PMC5693608
DOI:
10.1016/j.neuron.2017.10.011
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Elsevier Science Icon for PubMed Central
Loading ...
Support Center