Format

Send to

Choose Destination
Mol Metab. 2017 Nov;6(11):1407-1418. doi: 10.1016/j.molmet.2017.08.005. Epub 2017 Aug 18.

Identification of islet-enriched long non-coding RNAs contributing to β-cell failure in type 2 diabetes.

Author information

1
Department of Fundamental Neurosciences, Faculty of Biology and Medicine, University of Lausanne, Switzerland.
2
Montreal Diabetes Research Center and Centre de Recherche du CHUM, Montréal, Québec, Canada.
3
Department of Clinical Sciences-Malmö, Lund University Diabetes Centre, Lund University, Clinical Research Centre, SUS, Malmö, Sweden.
4
Pôle d'Endocrinologie, Diabète et Nutrition, Institut de Recherche Expérimentale et Clinique, Université Catholique de Louvain, Brussels, Belgium.
5
Garvan Institute of Medical Research, St Vincent's Hospital, University of New South Wales, Sydney Australia.
6
Vital-IT group, Swiss Institute of Bioinformatics, Lausanne, Switzerland.
7
Department of Fundamental Neurosciences, Faculty of Biology and Medicine, University of Lausanne, Switzerland. Electronic address: Romano.Regazzi@unil.ch.

Abstract

OBJECTIVE:

Non-coding RNAs constitute a major fraction of the β-cell transcriptome. While the involvement of microRNAs is well established, the contribution of long non-coding RNAs (lncRNAs) in the regulation of β-cell functions and in diabetes development remains poorly understood. The aim of this study was to identify novel islet lncRNAs differently expressed in type 2 diabetes models and to investigate their role in β-cell failure and in the development of the disease.

METHODS:

Novel transcripts dysregulated in the islets of diet-induced obese mice were identified by high throughput RNA-sequencing coupled with de novo annotation. Changes in the level of the lncRNAs were assessed by real-time PCR. The functional role of the selected lncRNAs was determined by modifying their expression in MIN6 cells and primary islet cells.

RESULTS:

We identified about 1500 novel lncRNAs, a number of which were differentially expressed in obese mice. The expression of two lncRNAs highly enriched in β-cells, βlinc2, and βlinc3, correlated to body weight gain and glycemia levels in obese mice and was also modified in diabetic db/db mice. The expression of both lncRNAs was also modulated in vitro in isolated islet cells by glucolipotoxic conditions. Moreover, the expression of the human orthologue of βlinc3 was altered in the islets of type 2 diabetic patients and was associated to the BMI of the donors. Modulation of the level of βlinc2 and βlinc3 by overexpression or downregulation in MIN6 and mouse islet cells did not affect insulin secretion but increased β-cell apoptosis.

CONCLUSIONS:

Taken together, the data show that lncRNAs are modulated in a model of obesity-associated type 2 diabetes and that variations in the expression of some of them may contribute to β-cell failure during the development of the disease.

KEYWORDS:

Diabetes; Gene expression; Insulin; Obesity; Pancreatic islet

Supplemental Content

Full text links

Icon for Elsevier Science Icon for PubMed Central
Loading ...
Support Center