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J Pharm Pharmacol. 2017 Nov 3. doi: 10.1111/jphp.12842. [Epub ahead of print]

Dose-dependent effect of triiodothyronine on the chondrogenic differentiation of mesenchymal stem cells from the bone marrow of female rats.

Author information

1
Departamento de Medicina Veterinária, Centro de Ciências Agrárias e Engenharias (CCAE) da Universidade Federal do Espírito Santo (UFES), Alegre, ES, Brazil.
2
Núcleo de Células-Tronco e Terapia Celular (NCT-TCA), Departamento de Clínica e Cirurgia Veterinárias, Escola de Veterinária da Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, MG, Brazil.
3
Laboratório de Imunologia Celular e Molecular, Departamento de Bioquímica e Imunologia, Instituto de Ciências Biológicas, UFMG, Belo Horizonte, MG, Brazil.
4
Departamento de Farmácia e Nutrição, Centro de Ciências Exatas, Naturais e da Saúde (CCENS) da UFES, Alegre, ES, Brazil.
5
Departamento de Biologia, Universidade Federal do Espírito Santo (UFES), Alegre, ES, Brazil.

Abstract

OBJECTIVES:

Verify the in-vitro effect of triiodothyronine (T3) on the chondrogenic differentiation of female rat bone marrow mesenchymal stem cells (BMMSCs) over several time periods and at several doses.

METHODS:

CD54 + /CD73 + /CD90 +  BMMSCs from Wistar female rats were cultured in chondrogenic medium with or without T3 (0.01; 1; 100; 1000 nm). At seven, 14 and 21 days, the cell morphology, chondrogenic matrix formation and expression of Sox9 and collagen II were evaluated.

KEY FINDINGS:

The dose of 100 nm did not alter the parameters evaluated in any of the periods studied. However, the 0.01 nm T3 dose improved the chondrogenic potential by increasing the chondrogenic matrix formation and expression of Sox9 and collagen II in at least one of the evaluated periods; the 1 nm T3 dose also improved the chondrogenic potential by increasing the chondrogenic matrix formation and the expression of collagen II in at least one of the evaluated periods. The 1000 nm T3 dose improved the chondrogenic potential by increasing the chondrogenic matrix formation and Sox9 expression in at least one of the evaluated periods.

CONCLUSIONS:

T3 has a dose-dependent effect on the differentiation of BMMSCs from female rats.

KEYWORDS:

bone marrow; chondrogenic differentiation; female rats; thyroid hormone

PMID:
29105086
DOI:
10.1111/jphp.12842
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