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PLoS One. 2017 Nov 3;12(11):e0187660. doi: 10.1371/journal.pone.0187660. eCollection 2017.

Intravital longitudinal wide-area imaging of dynamic bone marrow engraftment and multilineage differentiation through nuclear-cytoplasmic labeling.

Ahn S1,2, Choe K1,2, Lee S3, Kim K3, Song E1,2, Seo H1,2, Kim I3, Kim P1,2.

Author information

1
Graduate School of Nanoscience and Technology, Korea Advanced Institute of Science and Technology, Daejeon, Republic of Korea.
2
KI for Health Science and Technology (KIHST), Korea Advanced Institute of Science and Technology, Daejeon, Republic of Korea.
3
Graduate School of Medical Science and Engineering, Korea Advanced Institute of Science and Technology, Daejeon, Republic of Korea.

Abstract

Bone marrow is a vital tissue that produces the majority of erythrocytes, thrombocytes, and immune cells. Bone marrow transplantation (BMT) has been widely performed in patients with blood disorders and cancers. However, the cellular-level behaviors of the transplanted bone marrow cells over wide-areas of the host bone marrow after the BMT are not fully understood yet. In this work, we performed a longitudinal wide-area cellular-level observation of the calvarial bone marrow after the BMT in vivo. Using a H2B-GFP/β-actin-DsRed double-transgenic mouse model as a donor, a subcellular-level nuclear-cytoplasmic visualization of the transplanted bone marrow cells was achieved, which enabled a direct in vivo dynamic monitoring of the distribution and proliferation of the transplanted bone marrow cells. The same spots in the wide-area of the calvarial bone marrow were repeatedly identified using fluorescently labeled vasculature as a distinct landmark. It revealed various dynamic cellular-level behaviors of the transplanted BM cells in early stage such as cluster formation, migration, and active proliferation in vivo.

PMID:
29099870
PMCID:
PMC5669471
DOI:
10.1371/journal.pone.0187660
[Indexed for MEDLINE]
Free PMC Article

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