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J Proteome Res. 2018 Jan 5;17(1):129-140. doi: 10.1021/acs.jproteome.7b00431. Epub 2017 Nov 10.

Proteomic Analyses of Cysteine Redox in High-Fat-Fed and Fasted Mouse Livers: Implications for Liver Metabolic Homeostasis.

Author information

1
State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, College of Animal Science and Technology, Guangxi University , Nanning 530004, P.R. China.
2
Jingjie PTM Biolab Co. Ltd. , Hangzhou Economic and Technological Development Area, Hangzhou 310018, P.R. China.
3
Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, College of Animal Science and Veterinary Medicine, Huazhong Agricultural University , Wuhan, P.R. China.

Abstract

Intensive oxidative stress occurs during high-fat-diet-induced hepatic fat deposition, suggesting a critical role for redox signaling in liver metabolism. Intriguingly, evidence shows that fasting could also result in redox-profile changes largely through reduced oxidant or increased antioxidant levels. However, a comprehensive landscape of redox-modified hepatic substrates is lacking, thereby hindering our understanding of liver metabolic homeostasis. We employed a proteomic approach combining iodoacetyl tandem mass tag and nanoliquid chromatography tandem mass spectrometry to quantitatively probe the effects of high-fat feeding and fasting on in vivo redox-based cysteine modifications. Compared with control groups, ∼60% of cysteine residues exhibited downregulated oxidation ratios by fasting, whereas ∼94% of these ratios were upregulated by high-fat feeding. Importantly, in fasted livers, proteins exhibiting diminished cysteine oxidation were annotated in pathways associated with fatty acid metabolism, carbohydrate metabolism, insulin, peroxisome proliferator-activated receptors, and oxidative respiratory chain signaling, suggesting that fasting-induced redox changes targeted major metabolic pathways and consequently resulted in hepatic lipid accumulation.

KEYWORDS:

KEGG pathway; fasting; fatty acid metabolism; iodoTMT redox proteomics; oxidative respiratory chain

PMID:
29098862
DOI:
10.1021/acs.jproteome.7b00431
[Indexed for MEDLINE]

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