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Contrast Media Mol Imaging. 2017 Sep 26;2017:3981358. doi: 10.1155/2017/3981358. eCollection 2017.

Preliminary 19F-MRS Study of Tumor Cell Proliferation with 3'-deoxy-3'-fluorothymidine and Its Metabolite (FLT-MP).

Author information

1
Division of RI-Convergence Research, Korea Institute of Radiological & Medical Sciences, Seoul, Republic of Korea.
2
Convergence Institute of Biomedical Engineering and Biomaterials, Seoul National University of Science & Technology, Seoul, Republic of Korea.
3
Division of Medical Radiation Equipment, Korea Institute of Radiological & Medical Sciences, Seoul, Republic of Korea.
4
Department of Chemical & Biomolecular Engineering, Seoul National University of Science & Technology, Seoul, Republic of Korea.
5
Department of Nuclear Medicine, Korea Institute of Radiological & Medical Sciences, Seoul, Republic of Korea.

Abstract

The thymidine analogue 3'-deoxy-3'-[18F]fluorothymidine, or [18F]fluorothymidine ([18F]FLT), is used to measure tumor cell proliferation with positron emission tomography (PET) imaging technology in nuclear medicine. FLT is phosphorylated by thymidine kinase 1 (TK1) and then trapped inside cells; it is not incorporated into DNA. Imaging with 18F-radiolabeled FLT is a noninvasive technique to visualize cellular proliferation in tumors. However, it is difficult to distinguish between [18F]FLT and its metabolites by PET imaging, and quantification has not been attempted using current imaging methods. In this study, we successfully acquired in vivo19F spectra of natural or nonradioactive 3'-deoxy-3'-fluorothymidine ([19F]FLT) and its monophosphate metabolite (FLT-MP) in a tumor xenograft mouse model using 9.4T magnetic resonance imaging (MRI). This preliminary result demonstrates that 19F magnetic resonance spectroscopy (MRS) with FLT is suitable for the in vivo assessment of tumor aggressiveness and for early prediction of treatment response.

PMID:
29097919
PMCID:
PMC5634584
DOI:
10.1155/2017/3981358
[Indexed for MEDLINE]
Free PMC Article

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