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EBioMedicine. 2017 Nov;25:73-86. doi: 10.1016/j.ebiom.2017.09.021. Epub 2017 Sep 21.

Zika Virus Causes Persistent Infection in Porcine Conceptuses and may Impair Health in Offspring.

Author information

1
Vaccine and Infectious Disease Organization-International Vaccine Centre (VIDO-InterVac), University of Saskatchewan, Saskatoon, SK S7N 5E3, Canada.
2
Department of Physiology, Department of Obstetrics and Gynaecology, University of Toronto, Toronto, ON M5S 1A8, Canada.
3
Department of Small Animal Clinical Sciences, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, SK S7N 5B4, Canada.
4
Prairie Diagnostic Services, Saskatoon, SK S7N 5B4, Canada.
5
School of Public Health, University of Saskatchewan, Saskatoon, SK, Canada.
6
Vaccine and Infectious Disease Organization-International Vaccine Centre (VIDO-InterVac), University of Saskatchewan, Saskatoon, SK S7N 5E3, Canada; Department of Veterinary Microbiology, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, SK S7N 5B4, Canada.
7
Canada National Microbiology Laboratory, Public Health Agency of Canada, 1015 Arlington Street, Winnipeg, MB R3E 3R2, Canada.
8
Vaccine and Infectious Disease Organization-International Vaccine Centre (VIDO-InterVac), University of Saskatchewan, Saskatoon, SK S7N 5E3, Canada; Department of Veterinary Microbiology, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, SK S7N 5B4, Canada; School of Public Health, University of Saskatchewan, Saskatoon, SK, Canada. Electronic address: u.karniychuk@usask.ca.

Abstract

Outcomes of Zika virus (ZIKV) infection in pregnant women vary from the birth of asymptomatic offspring to abnormal development and severe brain lesions in fetuses and infants. There are concerns that offspring affected in utero and born without apparent symptoms may develop mental illnesses. Therefore, animal models are important to test interventions against in utero infection and health sequelae in symptomatic and likely more widespread asymptomatic offspring. To partially reproduce in utero infection in humans, we directly inoculated selected porcine conceptuses with ZIKV. Inoculation resulted in rapid trans-fetal infections, persistent infection in conceptuses, molecular pathology in fetal brains, fetal antibody and type I interferon responses. Offspring infected in utero showed ZIKV in their fetal membranes collected after birth. Some in utero affected piglets were small, depressed, had undersized brains, and showed seizures. Some piglets showed potentially increased activity. Our data suggest that porcine model of persistent in utero ZIKV infection has a strong potential for translational research and can be used to test therapeutic interventions in vivo.

KEYWORDS:

Behavior; Fetus; Offspring; Persistent infection; Pig; Zika virus

PMID:
29097124
PMCID:
PMC5704061
DOI:
10.1016/j.ebiom.2017.09.021
[Indexed for MEDLINE]
Free PMC Article

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