An ultrasensitive electrochemiluminescent immunosensor based on graphene oxide coupled graphite-like carbon nitride and multiwalled carbon nanotubes-gold for the detection of diclofenac

Biosens Bioelectron. 2018 Mar 15:101:260-267. doi: 10.1016/j.bios.2017.10.043. Epub 2017 Oct 19.

Abstract

In this study, a novel competition-type electrochemiluminescent (ECL) immunosensor for detecting diclofenac (DCF) was fabricated with graphene oxide coupled graphite-like carbon nitride (GO-g-C3N4) as signal probe for the first time. The ECL intensity of carboxylated g-C3N4 was significantly enhanced after being combined with graphene oxide (GO) which exhibited excellent charge-transport property. The sensing platform was constructed by multiwalled carbon nanotubes and gold nanoparticles (MWCNTs-AuNPs), which not only provided an effective matrix for immobilizing a large amount of coating antigen but also facilitated the electronic transmission rate to enhance the ECL intensity. Based on the synergistic effect of GO-g-C3N4 and MWCNTs-AuNPs composite, the proposed sensor showed high sensitivity, good stability, and wide linearity for the detection of DCF in the range of 0.005-1000ngmL-1 with a detection limit of 1.7pgmL-1. Furthermore, the developed immunoassay has been applied to real samples with satisfactory results. Therefore, this work provided a promising method for the detection of DCF and other small molecular compounds in the future.

Keywords: Competitive immunoassay; Diclofenac; Electrochemiluminescent immunosensor; Environmental analysis; GO-g-C(3)N(4); MWCNTs-AuNPs.

Publication types

  • Evaluation Study

MeSH terms

  • Biosensing Techniques / methods
  • Diclofenac / analysis*
  • Electrochemical Techniques / methods
  • Gold / chemistry*
  • Graphite / chemistry
  • Immunoassay / methods
  • Lakes / analysis
  • Limit of Detection
  • Luminescent Measurements / methods*
  • Metal Nanoparticles / chemistry*
  • Metal Nanoparticles / ultrastructure
  • Nanotubes, Carbon / chemistry*
  • Nanotubes, Carbon / ultrastructure
  • Nitriles / chemistry*
  • Oxides / chemistry
  • Wastewater / analysis
  • Water Pollutants, Chemical / analysis*

Substances

  • Nanotubes, Carbon
  • Nitriles
  • Oxides
  • Waste Water
  • Water Pollutants, Chemical
  • Diclofenac
  • cyanogen
  • Gold
  • Graphite