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Int J Syst Evol Microbiol. 2018 Jan;68(1):14-20. doi: 10.1099/ijsem.0.002293. Epub 2017 Nov 2.

Burkholderia puraquae sp. nov., a novel species of the Burkholderia cepacia complex isolated from hospital settings and agricultural soils.

Author information

1
1​CINDEFI, CONICET-CCT La Plata, Facultad de Ciencias Exactas, Universidad Nacional de La Plata, La Plata, Buenos Aires, Argentina.
2
‡​Present address: IBS, CONICET-CCT Nordeste, Facultad de Ciencias Exactas Químicas y Naturales, Universidad Nacional de Misiones, Posadas, Misiones, Argentina.
3
2​iBB-Institute for Bioengineering and Biosciences, Department of Bioengineering, Instituto Superior Técnico, Universidade de Lisboa, Av. Rovisco Pais, 1049-001 Lisboa, Portugal.
4
3​Hospital Santísima Trinidad de Córdoba, Córdoba, Argentina.
5
4​Fundación Instituto Leloir and IIBA-Consejo Nacional de Investigaciones Científicas y Tecnológicas, Buenos aires, Argentina.
6
5​IBBM, CONICET-CCT La Plata, Facultad de Ciencias Exactas, Universidad Nacional de La Plata, La Plata, Buenos Aires, Argentina.
7
6​Proteomics and Spectroscopy Unit (ZBS6) at the Centre for Biological Threats and Special Pathogens, Robert Koch-Institut, Berlin, Germany.
8
7​Sala de Microbiología, Hospital de Niños «Sor María Ludovica», La Plata, Buenos Aires, Argentina.
9
8​Laboratorio Cemar D. S. L. A. C, Municipalidad de Rosario, Santa Fe, Argentina.
10
9​IBS, CONICET-CCT Nordeste, Facultad de Ciencias Exactas Químicas y Naturales, Universidad Nacional de Misiones, Posadas, Misiones, Argentina.

Abstract

Bacteria from the Burkholderia cepacia complex (Bcc) are capable of causing severe infections in patients with cystic fibrosis (CF). These opportunistic pathogens are also widely distributed in natural and man-made environments. After a 12-year epidemiological surveillance involving Bcc bacteria from respiratory secretions of Argentinean patients with CF and from hospital settings, we found six isolates of the Bcc with a concatenated species-specific allele sequence that differed by more than 3 % from those of the Bcc with validly published names. According to the multilocus sequence analysis (MLSA), these isolates clustered with the agricultural soil strain, Burkholderia sp. PBP 78, which was already deposited in the PubMLST database. The isolates were examined using a polyphasic approach, which included 16S rRNA, recA, Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS), DNA base composition, average nucleotide identities (ANIs), fatty acid profiles, and biochemical characterizations. The results of the present study demonstrate that the seven isolates represent a single novel species within the Bcc, for which the name Burkholderia puraquae sp. nov. is proposed. Burkholderia puraquae sp. nov. CAMPA 1040T (=LMG 29660T=DSM 103137T) was designated the type strain of the novel species, which can be differentiated from other species of the Bcc mainly from recA gene sequence analysis, MLSA, ANIb, MALDI-TOF MS analysis, and some biochemical tests, including the ability to grow at 42 °C, aesculin hydrolysis, and lysine decarboxylase and β-galactosidase activities.

KEYWORDS:

Burkholderia cepacia complex; Burkholderia genus; Burkholderia puraquae sp. nov

PMID:
29095137
DOI:
10.1099/ijsem.0.002293
[Indexed for MEDLINE]

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