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Mol Oncol. 2017 Dec;11(12):1806-1825. doi: 10.1002/1878-0261.12151. Epub 2017 Nov 16.

Degradation of AMPK-α1 sensitizes BRAF inhibitor-resistant melanoma cells to arginine deprivation.

Author information

1
Department of Medicine, University of Miami Miller School of Medicine, FL, USA.
2
Sylvester Comprehensive Cancer Center, University of Miami Miller School of Medicine, FL, USA.
3
Division of Hematology and Oncology, Miami Veterans Affairs Healthcare System, FL, USA.
4
Dauer Electron Microscopy Lab, Department of Biology, University of Miami, FL, USA.
5
Department of Neurosurgery, Taipei Medical University-Wan Fang Hospital, Taiwan.
6
Graduate Institute of Clinical Medical Sciences, College of Medicine, Chang Gung University, Tao-Yuan, Taiwan.
7
Department of Surgery, University of Miami Miller School of Medicine, FL, USA.
8
Department of Molecular Pathology, University of Texas M. D. Anderson Cancer Center, Houston, TX, USA.
9
Department of Laboratory Medicine, Miami Veterans Affairs Healthcare System, FL, USA.

Abstract

Melanomas harboring BRAF mutation (V600E) are known to recur frequently following treatment with BRAF inhibitors (BRAFi) despite a high initial response rate. Our previous study has uncovered that BRAFi-resistant melanoma (BR) cells are vulnerable to arginine deprivation. It has been reported that naïve melanoma cells undergo autophagy and re-express argininosuccinate synthetase 1 (ASS1) to enable them to synthesize arginine for survival when encountering arginine deprivation. Abolishing these two factors in BR cells confers sensitivity to arginine deprivation. In this report, we further demonstrated that downregulation of AMPK-α1 in BR cells is a major factor contributing to impairment of autophagy as evidenced by decreased autophagosome formation. These BR cells also showed a metabolic shift from glucose to arginine dependence, which was supported by decreased expressions of GLUT1 (glucose transporter) and hexokinase II (HKII) coupled with less glucose uptake but high levels of arginine transporter CAT-2 expression. Furthermore, silencing CAT-2 expression also distinctly attenuated BR cell proliferation. Notably, when naïve melanoma cells became BR cells by long-term exposure to BRAFi, a stepwise degradation of AMPK-α1 was initiated via ubiquitin-proteasome system (UPS). We discovered that a novel E3 ligase, RING finger 44 (RNF44), is responsible for promoting AMPK-α1 degradation in BR cells. RNF44 expression in BR cells was upregulated by transcription factor CREB triggered by hyperactivation of ERK/AKT. High levels of RNF44 corresponding to low levels of AMPK-α1 appeared in BR xenografts and melanoma tumor samples from BR and BRAFi/MEK inhibitor (MEKi)-resistant (BMR) melanoma patients. Similar to BR cells, BMR cells were also sensitive to arginine deprivation. Our study provides a novel insight into the mechanism whereby BRAFi or BRAFi/MEKi resistance drives proteasomal degradation of AMPK-α1 and consequently regulates autophagy and metabolic reprogramming in melanoma cells.

KEYWORDS:

AMPK-α1; BRAF inhibitor-resistant melanoma; E3 ubiquitin ligase; autophagy; metabolic reprogramming; ubiquitin-proteasome system

PMID:
29094484
PMCID:
PMC5709618
DOI:
10.1002/1878-0261.12151
[Indexed for MEDLINE]
Free PMC Article

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