Format

Send to

Choose Destination
J Periodontal Implant Sci. 2017 Oct;47(5):273-291. doi: 10.5051/jpis.2017.47.5.273. Epub 2017 Oct 30.

Static magnetic fields promote osteoblastic/cementoblastic differentiation in osteoblasts, cementoblasts, and periodontal ligament cells.

Author information

1
Department of Oral and Maxillofacial Pathology, Institute of Oral Biology, Kyung Hee University School of Dentistry, Seoul, Korea.
2
Department of Dental Materials, Kyung Hee University School of Dentistry, Seoul, Korea.
3
Department of Biomaterials and Prosthodontics, Kyung Hee University Hospital at Gangdong, Kyung Hee University School of Dentistry, Seoul, Korea.

Abstract

Purpose:

Although static magnetic fields (SMFs) have been used in dental prostheses and osseointegrated implants, their biological effects on osteoblastic and cementoblastic differentiation in cells involved in periodontal regeneration remain unknown. This study was undertaken to investigate the effects of SMFs (15 mT) on the osteoblastic and cementoblastic differentiation of human osteoblasts, periodontal ligament cells (PDLCs), and cementoblasts, and to explore the possible mechanisms underlying these effects.

Methods:

Differentiation was evaluated by measuring alkaline phosphatase (ALP) activity, mineralized nodule formation based on Alizarin red staining, calcium content, and the expression of marker mRNAs assessed by reverse transcription polymerase chain reaction (RT-PCR). Signaling pathways were analyzed by western blotting and immunocytochemistry.

Results:

The activities of the early marker ALP and the late markers matrix mineralization and calcium content, as well as osteoblast- and cementoblast-specific gene expression in osteoblasts, PDLCs, and cementoblasts were enhanced. SMFs upregulated the expression of Wnt proteins, and increased the phosphorylation of glycogen synthase kinase-3β (GSK-3β) and total β-catenin protein expression. Furthermore, p38 and c-Jun N-terminal kinase (JNK) mitogen-activated protein kinase (MAPK), and nuclear factor-κB (NF-κB) pathways were activated.

Conclusions:

SMF treatment enhanced osteoblastic and/or cementoblastic differentiation in osteoblasts, cementoblasts, and PDLCs. These findings provide a molecular basis for the beneficial osteogenic and/or cementogenic effect of SMFs, which could have potential in stimulating bone or cementum formation during bone regeneration and in patients with periodontal disease.

KEYWORDS:

Bone regeneration; Periodontal guided tissue regeneration; Relative biological effectiveness; Signal transduction; Wnt proteins

Supplemental Content

Full text links

Icon for Korean Academy of Periodontology Icon for PubMed Central
Loading ...
Support Center