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Chin Med. 2017 Oct 24;12:30. doi: 10.1186/s13020-017-0151-z. eCollection 2017.

Effects of pretreatment with methanol extract of Peucedani Radix on transient ischemic brain injury in mice.

Author information

1
College of Korean Medicine, Dong-Eui University, Yangjeong-ro, Busanjin-gu, Busan, 47227 Republic of Korea.
2
School of Korean Medicine, Pusan National University, Yangsan, Gyeongnam 50612 Republic of Korea.
3
School of Public Health, Far East University, Chungbuk, 27601 Republic of Korea.
4
College of Medicine, Dongguk University, Ilsandong-gu, Gyeonggi-do 10326 Republic of Korea.
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Contributed equally

Abstract

BACKGROUND:

Stroke is the second most common cause of death and may result in various disabilities; thus, identification of neuroprotective therapeutic agents is important. Peucedani Radix (PR), the root of Angelica decursiva, is a well-known remedy for damp and phlegm in Korean medicine and has also been shown to exert antioxidant and anti-inflammatory activities. This study was performed to investigate the mechanism underlying the anti-inflammatory effect of methanol extract of PR (PRex) on cerebral ischemic injury.

METHODS:

C57BL/6 male mice were orally administered PRex (20, 60, or 200 mg/kg) at 2 days, 1 day, and 1 h prior to middle cerebral artery occlusion (MCAO). Twenty-four hours after MCAO, the infarct volume was measured and the neurological deficit score was assessed. The inflammatory-related substances in the ipsilateral hemisphere were determined by western blotting, DCFH-DA assay, TBARS assay, and ELISA.

RESULTS:

PRex pretreatment significantly decreased the infarct volume at 24 h after MCAO. Moreover, PRex effectively suppressed the expression of iNOS, ROS, MDA, and pro-inflammatory cytokines, such as IL-1β and TNF-α, in brain tissue of mice with MCAO-induced brain injury.

CONCLUSIONS:

PRex protected neurons from ischemic brain injury in mice through its antioxidant and anti-inflammatory activities. Our results suggested that PR could be a promising candidate in the therapy of ischemia-induced brain damage.

KEYWORDS:

Angelica decursiva; Anti-inflammation; Peucedani Radix; Stroke

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