Format

Send to

Choose Destination
Genes Dev. 2017 Oct 1;31(19):1927-1932. doi: 10.1101/gad.304113.117.

Genomic imprinting of Xist by maternal H3K27me3.

Inoue A1,2,3, Jiang L1,2,3, Lu F1,2,3, Zhang Y1,2,3,4,5.

Author information

1
Howard Hughes Medical Institute, Boston Children's Hospital, Boston, Massachusetts 02115, USA.
2
Program in Cellular and Molecular Medicine, Boston Children's Hospital, Boston, Massachusetts 02115, USA.
3
Division of Hematology/Oncology, Department of Pediatrics, Boston Children's Hospital, Boston, Massachusetts 02115, USA.
4
Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115, USA.
5
Harvard Stem Cell Institute, Boston, Massachusetts 02115, USA.

Abstract

Maternal imprinting at the Xist gene is essential to achieve paternal allele-specific imprinted X-chromosome inactivation (XCI) in female mammals. However, the mechanism underlying Xist imprinting is unclear. Here we show that the Xist locus is coated with a broad H3K27me3 domain that is established during oocyte growth and persists through preimplantation development in mice. Loss of maternal H3K27me3 induces maternal Xist expression and maternal XCI in preimplantation embryos. Our study thus identifies maternal H3K27me3 as the imprinting mark of Xist.

KEYWORDS:

H3K27me3; X-chromosome inactivation; genomic imprinting; mouse early development

PMID:
29089420
PMCID:
PMC5710138
DOI:
10.1101/gad.304113.117
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for HighWire Icon for PubMed Central
Loading ...
Support Center