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Int J Mol Sci. 2017 Oct 31;18(11). pii: E2286. doi: 10.3390/ijms18112286.

Efficient Generation of Genome-Modified Mice Using Campylobacter jejuni-Derived CRISPR/Cas.

Author information

1
Department of Animal Resource Sciences, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1, Yayoi, Tokyo 113-8657, Japan. awtrfj@mail.ecc.u-tokyo.ac.jp.
2
Department of Animal Resource Sciences, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1, Yayoi, Tokyo 113-8657, Japan. ikeda-arisa228@g.ecc.u-tokyo.ac.jp.
3
Department of Animal Resource Sciences, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1, Yayoi, Tokyo 113-8657, Japan. aks@mail.ecc.u-tokyo.ac.jp.
4
Department of Animal Resource Sciences, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1, Yayoi, Tokyo 113-8657, Japan. aknaito@mail.ecc.u-tokyo.ac.jp.

Abstract

Mammalian zygote-mediated genome-engineering by Clustered Regularly Interspaced Short Palindromic Repeat (CRISPR)/Cas is currently used for the generation of genome-modified animals. Here, we report that a Campylobacter jejuni-derived orthologous CRISPR/Cas system recognizes a 5'-NNNVRYAC sequence as a protospacer-adjacent motif in mouse zygotes, and is applicable for efficient generation of knockout mice. Moreover, this novel CRISPR/Cas can be used for zygote-mediated knock-in at a unique locus, suggesting that this system could help to expand the feasibility of the zygote-mediated generation of genome-modified animals.

KEYWORDS:

CRISPR/Cas; Campylobacter jejuni; genome-modified mouse

PMID:
29088065
PMCID:
PMC5713256
DOI:
10.3390/ijms18112286
[Indexed for MEDLINE]
Free PMC Article

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