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Cell Microbiol. 2018 Apr;20(4). doi: 10.1111/cmi.12796. Epub 2018 Jan 16.

Proteomics analysis of Medicago truncatula response to infection by the phytopathogenic bacterium Ralstonia solanacearum points to jasmonate and salicylate defence pathways.

Author information

1
Department of Plant Breeding and Biotechnology, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran.
2
EcoLab, Université de Toulouse, CNRS, INPT, UPS, Toulouse, France.
3
Universite de Toulouse, IFR40, Plateforme Protéomique du Génopole Toulouse Midi-Pyrénées, Institut de Pharmacologie et de Biologie Structurale, CNRS UMR 5089, 31077, Toulouse, France.
4
Department of Agricultural biotechnology, College of Agriculture, Isfahan University of Technology, 84156-83111, Isfahan, Iran.

Abstract

The infection of the model legume Medicago truncatula with Ralstonia solanacearum GMI1000 gives rise to bacterial wilt disease via colonisation of roots. The root and leaf responses to early infection (1 and 3 days post infection) were characterised to investigate the molecular mechanisms of plant resistance or susceptibility. A proteomics approach based on pools of susceptible and resistant recombinant inbred lines was used to specifically target the mechanisms for tolerance. Differential abundances were evidenced for proteins involved in defence (e.g., PR5, PR10, or Kunitz protease inhibitors) and signalling pathways (such as cyclophilin). R. solanacearum inoculation modifies expression levels of those genes, either in both genotypes (AOS1, LOX4, and proteinase inhibitors) or specifically in the resistant line (PR proteins). Exogenous application of salicylic acid (SA) enhanced tolerance to the bacteria, whereas methyl jasmonate (MeJA) enhanced short-term tolerance then promoted disease in the susceptible ecotype, suggesting that they may mediate defence responses. Conversely, proteomics-identified genes were also shown to be SA or MeJA responsive. This is the first description of differential response to R. solanacearum in M. truncatula. Our results suggest that root basal defence is activated at 1 dpi, together with the JA pathway. Specific resistance is then evidenced at three dpi, with the up-regulation of SA-dependent PR proteins.

KEYWORDS:

RILs; barrel medic; hydroponic culture; proteomics; root disease

PMID:
29084417
DOI:
10.1111/cmi.12796
[Indexed for MEDLINE]

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