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Biomed Opt Express. 2017 Sep 27;8(10):4742-4755. doi: 10.1364/BOE.8.004742. eCollection 2017 Oct 1.

Label free monitoring of megakaryocytic development and proplatelet formation in vitro.

Author information

1
Department of Biomedical Engineering, Tufts University, 4 Colby St., 02155 Medford MA, USA.
2
These authors contributed equally to this work.
3
Department of Molecular Medicine, University of Pavia, Pavia, Italy.
4
Biotechnology Research Laboratories, IRCCS San Matteo Foundation, Pavia, Italy.

Abstract

Megakaryopoiesis and platelet production are complex biological processes that require tight regulation of successive lineage commitment steps and are ultimately responsible for maintaining and renewing the pool of circulating platelets in the blood. Despite major advancements in the understanding of megakaryocytic biology, the detailed mechanisms driving megakaryocytic differentiation have yet to be elucidated. Here we show that automated image analysis algorithms applied to two-photon excited fluorescence (TPEF) images can non-invasively monitor structural and metabolic megakaryocyte behavior changes occurring during differentiation and platelet formation in vitro. Our results demonstrate that high-contrast, label-free two photon imaging holds great potential in studying the underlying physiological processes controlling the intricate process of platelet production.

KEYWORDS:

(170.0170) Medical optics and biotechnology; (170.1470) Blood or tissue constituent monitoring; (170.3880) Medical and biological imaging; (180.2520) Fluorescence microscopy; (190.0190) Nonlinear optics

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