Format

Send to

Choose Destination
Biomed Opt Express. 2017 Sep 11;8(10):4427-4437. doi: 10.1364/BOE.8.004427. eCollection 2017 Oct 1.

Label-free sensing of the binding state of MUC1 peptide and anti-MUC1 aptamer solution in fluidic chip by terahertz spectroscopy.

Author information

1
Department of Laboratory Medicine, Southwest Hospital, Third Military Medical University, Chongqing 400038, China.
2
Contributed equally.
3
Chongqing Key laboratory of Multi-Scale Manufacturing Technology, Chongqing Institute of Green and Intelligent Technology, Chinese Academy of Sciences, Chongqing, 400714, China.
4
hcui@cigit.ac.cn.
5
weiling_fu@126.com.

Abstract

The aptamer and target molecule binding reaction has been widely applied for construction of aptasensors, most of which are labeled methods. In contrast, terahertz technology proves to be a label-free sensing tool for biomedical applications. We utilize terahertz absorption spectroscopy and molecular dynamics simulation to investigate the variation of binding-induced collective vibration of hydrogen bond network in a mixed solution of MUC1 peptide and anti-MUC1 aptamer. The results show that binding-induced alterations of hydrogen bond numbers could be sensitively reflected by the variation of terahertz absorption coefficients of the mixed solution in a customized fluidic chip. The minimal detectable concentration is determined as 1 pmol/μL, which is approximately equal to the optimal immobilized concentration of aptasensors.

KEYWORDS:

(170.1420) Biology; (280.1415) Biological sensing and sensors; (300.6495) Spectroscopy, terahertz

Supplemental Content

Full text links

Icon for PubMed Central
Loading ...
Support Center