Optical imaging for the assessment of hepatocyte metabolic state in ischemia and reperfusion injuries

Mette F la Cour; Shima Mehrvar; Joohyun Kim; Alicia Martin; Michael A Zimmerman; Johnny C Hong; Mahsa Ranji

doi:10.1364/BOE.8.004419

Optical imaging for the assessment of hepatocyte metabolic state in ischemia and reperfusion injuries

Biomed Opt Express. 2017 Sep 8;8(10):4419-4426. doi: 10.1364/BOE.8.004419. eCollection 2017 Oct 1.

Authors

Mette F la Cour^{1

2}, Shima Mehrvar^{1

2}, Joohyun Kim³, Alicia Martin³, Michael A Zimmerman³, Johnny C Hong³, Mahsa Ranji¹

Affiliations

¹ Biopotonics Lab, Electrical Engineering Department, University of Wisconsin - Milwaukee, 3200 N Cramer St, Milwaukee, WI 53211, USA.
² Both contributed equally and are therefore first authors.
³ Division of Transplant Surgery, Department of Surgery, Medical College of Wisconsin, 9200 W. Wisconsin Avenue, Suite E5700, Milwaukee, WI 53226, USA.

Abstract

Deterioration in mitochondrial function leads to hepatic ischemia and reperfusion injury (IRI) in liver surgery and transplantation. 3D optical cryoimaging was used to measure the levels of mitochondrial coenzymes NADH and FAD, and their redox ratio (NADH/FAD) gave a quantitative marker for hepatocyte oxidative stress during IRI. Using a rat model, five groups were compared: control, ischemia for 60 or 90 minutes (Isc60, Isc90), ischemia for 60 or 90 minutes followed by reperfusion of 24 hours (IRI60, IRI90). Ischemia alone did not cause a significant increase in the redox ratio; however, the redox ratio in both IRI60 and IRI90 groups was significantly decreased by 29% and 71%, respectively. A significant correlation was observed between the redox ratio and other markers of injury such as serum aminotransferase levels and the tissue ATP level. The mitochondrial redox state can be successfully measured using optical cryoimaging as a quantitative marker of hepatic IR injury.

Keywords: (000.1430) Biology and medicine; (100.2960) Image analysis; (170.0110) Imaging systems; (170.3880) Medical and biological imaging; (170.6280) Spectroscopy, fluorescence and luminescence; (260.2510) Fluorescence.