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Biomed Opt Express. 2017 Sep 7;8(10):4369-4379. doi: 10.1364/BOE.8.004369. eCollection 2017 Oct 1.

Rapid adaptive remote focusing microscope for sensing of volumetric neural activity.

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Centre for Neural Circuits and Behaviour, University of Oxford, Mansfield Road, Oxford OX1 3SR, UK.
Department of Engineering Science, University of Oxford, Parks Road, Oxford OX1 3PJ, UK.


The ability to record neural activity in the brain of a living organism at cellular resolution is of great importance for defining the neural circuit mechanisms that direct behavior. Here we present an adaptive two-photon microscope optimized for extraction of neural signals over volumes in intact Drosophila brains, even in the presence of specimen motion. High speed volume imaging was made possible through reduction of spatial resolution while maintaining the light collection efficiency of a high resolution, high numerical aperture microscope. This enabled simultaneous recording of odor-evoked calcium transients in a defined volume of mushroom body Kenyon cell bodies in a live fruit fly.


(000.1430) Biology and medicine; (110.0180) Microscopy; (110.1080) Active or adaptive optics; (170.6900) Three-dimensional microscopy

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