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Angew Chem Int Ed Engl. 2018 Apr 9;57(16):4329-4337. doi: 10.1002/anie.201709201. Epub 2018 Feb 28.

Genomes in Focus: Development and Applications of CRISPR-Cas9 Imaging Technologies.

Knight SC1, Tjian R2,3,4,5, Doudna JA1,2,3,4,6,7.

Author information

1
Department of Chemistry, University of California, Berkeley, Berkeley, CA, USA.
2
Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA, USA.
3
Howard Hughes Medical Institute, USA.
4
Li Ka Shing Biomedical and Health Sciences Center, University of California, Berkeley, Berkeley, CA, USA.
5
CIRM Center of Excellence, University of California, Berkeley, Berkeley, CA, USA.
6
MBIB Division, Lawrence Berkeley National Laboratory, Berkeley, CA, USA.
7
Innovative Genomics Institute, University of California, Berkeley, Berkeley, CA, USA.

Abstract

The discovery of the CRISPR-Cas9 endonuclease has enabled facile genome editing in living cells and organisms. Catalytically inactive Cas9 (dCas9) retains the ability to bind DNA in an RNA-guided fashion, and has additionally been explored as a tool for transcriptional modulation, epigenetic editing, and genome imaging. This Review highlights recent progress and challenges in the development of dCas9 for imaging genomic loci. The emergence and maturation of this technology offers the potential to answer mechanistic questions about chromosome dynamics and three-dimensional genome organization in vivo.

KEYWORDS:

3D genome organization; CRISPR; genome editing; imaging; microscopy

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