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J Gen Virol. 2018 Feb;99(2):258-264. doi: 10.1099/jgv.0.000949. Epub 2017 Oct 27.

Culex quinquefasciatus mosquitoes do not support replication of Zika virus.

Author information

1
1​Department of Virology, Arboviruses and Insect Vectors, Institut Pasteur, Paris, France.
2
2​Instituto Oswaldo Cruz, Rio de Janeiro, Brazil.
3
3​Departamento de Bioquímica e Imunologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, 6627-Pampulha-Belo Horizonte-MG, CEP 31270-901, Brazil.
4
4​MRC-University of Glasgow Centre for Virus Research, Glasgow G61 1QH, Scotland, UK.
5
†​Present address: University of Reunion Island, UMR PIMIT (Processus Infectieux en Milieu Insulaire Tropical), CNRS 9192, INSERM U1187, IRD 249, Sainte-Clotilde, Reunion Island, France.

Abstract

The rapid spread of Zika virus (ZIKV) in the Americas raised many questions about the role of Culex quinquefasciatus mosquitoes in transmission, in addition to the key role played by the vector Aedes aegypti. Here we analysed the competence of Cx. quinquefasciatus (with or without Wolbachia endosymbionts) for a ZIKV isolate. We also examined the induction of RNA interference pathways after viral challenge and the production of small virus-derived RNAs. We did not observe any infection nor such small virus-derived RNAs, regardless of the presence or absence of Wolbachia. Thus, Cx. quinquefasciatus does not support ZIKV replication and Wolbachia is not involved in producing this phenotype. In short, these mosquitoes are very unlikely to play a role in transmission of ZIKV.

KEYWORDS:

Culex spp. mosquitoes; Zika virus; mosquito; replication; transmission

PMID:
29076805
PMCID:
PMC5882084
DOI:
10.1099/jgv.0.000949
[Indexed for MEDLINE]
Free PMC Article

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