Format

Send to

Choose Destination
J Cell Sci. 2018 Jan 29;131(2). pii: jcs203331. doi: 10.1242/jcs.203331.

Phragmoplast microtubule dynamics - a game of zones.

Author information

1
Institute of Biological Chemistry, Pullman, WA 99164, USA andrei.smertenko@wsu.edu.
2
Molecular Plant Sciences Graduate Program, Washington State University, Pullman, WA 99164, USA.
3
Department of Horticulture, Washington State University, Pullman, WA 99164, USA.
4
Department of Crop and Soil Sciences, Washington State University, Pullman, WA 99164, USA.
5
Institute of Biological Chemistry, Pullman, WA 99164, USA.
6
School of Biological Sciences, Washington State University, Pullman, WA 99164, USA.
7
Department of Plant Pathology, Washington State University, Pullman, WA 99164, USA.

Abstract

Plant morphogenesis relies on the accurate positioning of the partition (cell plate) between dividing cells during cytokinesis. The cell plate is synthetized by a specialized structure called the phragmoplast, which consists of microtubules, actin filaments, membrane compartments and associated proteins. The phragmoplast forms between daughter nuclei during the transition from anaphase to telophase. As cells are commonly larger than the originally formed phragmoplast, the construction of the cell plate requires phragmoplast expansion. This expansion depends on microtubule polymerization at the phragmoplast forefront (leading zone) and loss at the back (lagging zone). Leading and lagging zones sandwich the 'transition' zone. A population of stable microtubules in the transition zone facilitates transport of building materials to the midzone where the cell plate assembly takes place. Whereas microtubules undergo dynamic instability in all zones, the overall balance appears to be shifted towards depolymerization in the lagging zone. Polymerization of microtubules behind the lagging zone has not been reported to date, suggesting that microtubule loss there is irreversible. In this Review, we discuss: (1) the regulation of microtubule dynamics in the phragmoplast zones during expansion; (2) mechanisms of the midzone establishment and initiation of cell plate biogenesis; and (3) signaling in the phragmoplast.

KEYWORDS:

Cell plate; Cytokinesis; Microtubule dynamics; Phragmoplast

PMID:
29074579
PMCID:
PMC6518215
DOI:
10.1242/jcs.203331
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for HighWire Icon for PubMed Central
Loading ...
Support Center