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FEMS Microbiol Ecol. 2017 Nov 1;93(11). doi: 10.1093/femsec/fix144.

Links between seawater flooding, soil ammonia oxidiser communities and their response to changes in salinity.

Author information

1
Institute of Biological and Environmental Sciences, University of Aberdeen, Cruickshank Building, Aberdeen AB24 3UU, UK.
2
Department of Genomic and Applied Microbiology and Göttingen Genomics Laboratory, Institute of Microbiology and Genetics, Georg-August-University Göttingen, Grisebachstr. 8, D-37077 Göttingen, Germany.
3
Max Planck Institute for Biogeochemistry, Hans-Knöll-Str. 10, D-07745 Jena, Germany.
4
Department of Sedimentology and Environmental Geology, Geoscience Center, Georg-August-University Göttingen, Goldschmidtstr. 3, D-37077 Göttingen, Germany.

Abstract

Coastal areas worldwide are challenged by climate change-associated increases in sea level and storm surge quantities that potentially lead to more frequent flooding of soil ecosystems. Currently, little is known of the effects of inundation events on microorganisms controlling nitrification in these ecosystems. The goal of this study was to investigate the impact of seawater flooding on the abundance, community composition and salinity tolerance of soil ammonia oxidisers. Topsoil was sampled from three islands flooded at different frequencies by the Wadden Sea. Archaeal ammonia oxidiser amoA genes were more abundant than their betaproteobacterial counterparts, and the distribution of archaeal and bacterial ammonia oxidiser amoA and 16S rRNA gene sequences significantly differed between the islands. The findings indicate selection of ammonia oxidiser phylotypes with greater tolerance to high salinity and slightly alkaline pH (e.g. Nitrosopumilus representatives) in frequently flooded soils. A cluster phylogenetically related to gammaproteobacterial ammonia oxidisers was detected in all samples analysed in this survey. Nevertheless, no gammaprotebacterial amoA genes could be amplified via PCR and only betaproteobacterial ammonia oxidisers were detected in enrichment cultures. A slurry-based experiment demonstrated the tolerance of both bacterial and archaeal ammonia oxidisers to a wide range of salinities (e.g. Wadden Sea water salinity) in soil naturally exposed to seawater at a high frequency.

KEYWORDS:

Nitrosococcus; ammonia-oxidising archaea; ammonia-oxidising bacteria; flooding; nitrification; salinity

PMID:
29069386
DOI:
10.1093/femsec/fix144
[Indexed for MEDLINE]

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