Protective efficacy of antidiarrheal agents in a permeability model of Escherichia coli-infected CacoGoblet® cells

Future Microbiol. 2017 Dec:12:1449-1455. doi: 10.2217/fmb-2016-0195. Epub 2017 Oct 25.

Abstract

Aim: To compare the protective efficacy of gelatine tannate/probiotic with other antidiarrheal agents in Escherichia coli-inoculated CacoGoblet® cells.

Methods: Four test compounds - gelatine tannate plus inactivated probiotic, diosmectite, probiotic mixture and Saccharomyces boulardii - were added to E. coli-infected CacoGoblet cells. After 1 and 24 h, transepithelial electrical resistance was measured and a lucifer yellow assay performed.

Results: Gelatine tannate/probiotic markedly increased transepithelial electrical resistance by 123.1% (at 1 h) and 149.5% (at 24 h), and produced paracellular flux values of 0.41% (1 h) and 1.34% (24 h), which were considerably less than the E. coli-invasion value (2.41%).

Conclusion: The protective efficacy of gelatine tannate/probiotic against E. coli-induced reduction of membrane integrity manifests early and is maintained for 24 h.

Keywords: Escherichia coli; acute gastroenteritis; diarrhea; gelatin tannate; inactivated probiotics; mucoprotectors.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antidiarrheals / pharmacology*
  • Cell Line
  • Diarrhea / drug therapy*
  • Diarrhea / microbiology
  • Epithelial Cells / microbiology
  • Epithelial Cells / physiology
  • Escherichia coli Infections / drug therapy*
  • Escherichia coli Infections / microbiology
  • Gelatin / pharmacology
  • Goblet Cells / microbiology
  • Goblet Cells / physiology
  • Humans
  • Models, Biological*
  • Permeability*
  • Probiotics / pharmacology*
  • Silicates / pharmacology
  • Tannins / pharmacology

Substances

  • Antidiarrheals
  • Silicates
  • Tannins
  • tannate gelatin
  • Gelatin
  • Smectite