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Benef Microbes. 2018 Feb 27;9(2):333-344. doi: 10.3920/BM2017.0096. Epub 2017 Oct 25.

Bacteriocin biosynthesis contributes to the anti-inflammatory capacities of probiotic Lactobacillus plantarum.

Author information

1
1 Department of Food Science and Technology, University of California, One Shields Avenue, Davis, CA 95616, USA.
2
2 Department of Plant Pathology, University of California, One Shields Avenue, Davis CA 95616-8751, USA.
3
3 Bayer U.S. LLC, Crop Science Division, 890 Embarcadero Dr, West Sacramento, CA 95605, USA.
4
4 Comparative Pathology Laboratory, School of Veterinary Medicine, University of California, 944 Garrod Dr. 2045 Davis, CA 95616, USA.

Abstract

Plantaricin EF (PlnEF) is a class IIb bacteriocin produced by Lactobacillus plantarum. We compared L. plantarum NCIMB8826 and LM0419, a plnEFI deletion mutant of that strain lacking plnEF and the gene for the cognate immunity protein plnI, in a 2,4,6-trinitrobenzenesulfonic acid (TNBS) induced mouse model of acute inflammatory bowel disease. Mice fed either L. plantarum NCIMB8826 or LM0419 were not protected against TNBS according to either disease activity or histology (Ameho) scores. Mice consuming NCIMB8826 exhibited intermediate (non-significant) levels of colonic tumour necrosis factor alpha (TNF-α) and interleukin-6 (IL-6) that ranged between the TNBS-treated animals and healthy controls. By comparison, TNF-α and IL-6 quantities were elevated in mice given L. plantarum LM0419 and equivalent to mice given TNBS alone. Both strains survived digestive tract transit in equal numbers and did not result in global changes to the bacterial composition in the intestine according to 16S rRNA gene sequencing either prior to or after TNBS administration. Examination of intestinal taxa showed that mice consuming wild-type L. plantarum, but not LM0419 contained lower proportions of Mucispirillum (Deferribacteres phylum) in the faeces prior to TNBS administration and Parabacteroides (Bacteroidetes phylum) in the caecum after disease induction. Parabacteroides also positively correlated with disease activity and histology scores. These findings suggest a role for PlnEFI production by L. plantarum in benefiting digestive tract health.

KEYWORDS:

Lactobacillus; bacteriocin; gut microbiota; intestinal inflammation; probiotics

PMID:
29065706
DOI:
10.3920/BM2017.0096
[Indexed for MEDLINE]

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