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PLoS One. 2017 Oct 24;12(10):e0186938. doi: 10.1371/journal.pone.0186938. eCollection 2017.

Drosophila VAMP7 regulates Wingless intracellular trafficking.

Gao H1,2, He F2,3, Lin X4,5, Wu Y3,6.

Author information

1
School of Life Sciences, University of Science and Technology of China, Hefei, China.
2
State Key Laboratory of Membrane Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China.
3
University of Chinese Academy of Sciences, Beijing, China.
4
State Key Laboratory of Genetic Engineering, Institute of Genetics, Collaborative Innovation Center of Genetics and Development, School of Life Sciences, Fudan University, Shanghai, China.
5
Division of Developmental Biology, Cincinnati Children's Hospital Medical Center, Cincinnati, OH, United States of America.
6
State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China.

Abstract

Drosophila Wingless (Wg) is a morphogen that determines cell fate during development. Previous studies have shown that endocytic pathways regulate Wg trafficking and signaling. Here, we showed that loss of vamp7, a gene required for vesicle fusion, dramatically increased Wg levels and decreased Wg signaling. Interestingly, we found that levels of Dally-like (Dlp), a glypican that can interact with Wg to suppress Wg signaling at the dorsoventral boundary of the Drosophila wing, were also increased in vamp7 mutant cells. Moreover, Wg puncta in Rab4-dependent recycling endosomes were Dlp positive. We hypothesize that VAMP7 is required for Wg intracellular trafficking and the accumulation of Wg in Rab4-dependent recycling endosomes might affect Wg signaling.

PMID:
29065163
PMCID:
PMC5655445
DOI:
10.1371/journal.pone.0186938
[Indexed for MEDLINE]
Free PMC Article

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