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Genet Mol Biol. 2017 Oct-Dec;40(4):813-823. doi: 10.1590/1678-4685-GMB-2016-0222. Epub 2017 Oct 23.

Mapping QTLs for drought tolerance in a SEA 5 x AND 277 common bean cross with SSRs and SNP markers.

Author information

1
Centro de Recursos Genéticos Vegetais, Instituto Agronômico (IAC), Campinas, SP, Brazil.
2
Ciências Biológicas, Universidade Tecnológica Federal do Paraná (UTFPR), Dois Vizinhos, PR, Brazil.
3
Centro de Grãos e Fibras, Instituto Agronômico (IAC), Campinas, SP, Brazil.
4
Departamento de Agronomia, Universidade Estadual de Maringá (UEM), Maringá, PR, Brazil.
5
Department of Agriculture and Natural Sciences, Tennessee State University, Nashville, TN, USA.
6
Bean Program, Centro Nacional de Pesquisas Arroz e Feijão, Goiânia, GO, Brazil.

Abstract

The common bean is characterized by high sensitivity to drought and low productivity. Breeding for drought resistance in this species involves genes of different genetic groups. In this work, we used a SEA 5 x AND 277 cross to map quantitative trait loci associated with drought tolerance in order to assess the factors that determine the magnitude of drought response in common beans. A total of 438 polymorphic markers were used to genotype the F8 mapping population. Phenotyping was done in two greenhouses, one used to simulate drought and the other to simulate irrigated conditions. Fourteen traits associated with drought tolerance were measured to identify the quantitative trait loci (QTLs). The map was constructed with 331 markers that covered all 11 chromosomes and had a total length of 1515 cM. Twenty-two QTLs were discovered for chlorophyll, leaf and stem fresh biomass, leaf biomass dry weight, leaf temperature, number of pods per plant, number of seeds per plant, seed weight, days to flowering, dry pod weight and total yield under well-watered and drought (stress) conditions. All the QTLs detected under drought conditions showed positive effects of the SEA 5 allele. This study provides a better understanding of the genetic inheritance of drought tolerance in common bean.

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