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J Gen Microbiol. 1988 Jun;134(6):1611-21.

Molecular cloning of a gene affecting the autolysin level and flagellation in Bacillus subtilis.

Author information

1
Department of Applied Biology, Faculty of Textile Science and Technology, Shinshu University, Nagano, Japan.

Abstract

A 2.8 kb PstI fragment of Bacillus subtilis 168W DNA has been cloned into Escherichia coli HB101 and B. subtilis AG5 using pAC3 as a shuttle plasmid. The new plasmid (pBRG1), of 10.2 kb, complemented flaD mutations which show reduced production of autolysin(s), filamentation and non-motility (deficiency of flagella). Deletion experiments showed that the suppressive gene is located between the HindIII and XbaI sites (1.0 kb apart) in pBRG1. The integration of a plasmid having chloramphenicol resistance closely linked to the flaD gene into the B. subtilis AC703 chromosome and its genetic analysis indicated that the cloned fragment contained the flaD gene itself. A high-copy-number plasmid carrying the cloned gene did not lead to an increase in autolysin production above the wild-type level, but it changed the colony morphology from smooth to rough. Among several autolysin-deficient mutations, lyt-151 was suppressed only by the high-copy-number plasmid carrying the cloned gene.

PMID:
2906087
DOI:
10.1099/00221287-134-6-1611
[Indexed for MEDLINE]

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