Format

Send to

Choose Destination
Cell Physiol Biochem. 2017;43(5):2088-2101. doi: 10.1159/000484193. Epub 2017 Oct 23.

Cannabinoids Regulate the Diameter of Pericyte-Containing Retinal Capillaries in Rats.

Zong Y1,2, Zhou X3,2, Cheng J1,2, Yu J1,2, Wu J3,2, Jiang C1,2,4.

Author information

1
Department of Ophthalmology and Vision Science, Eye and ENT Hospital, Fudan University, Shanghai, China.
2
Key Laboratory of Myopia of State Health Ministry, and Key Laboratory of Visual Impairment and Restoration of Shanghai, Shanghai, China.
3
Eye and ENT Hospital, State Key laboratory of Medical Neurobiology, Institutes of Brain Science and Collaborative Innovation Center for Brain Science, Fudan University, Shanghai, China.
4
Department of Ophthalmology, People's Hospital of Shanghai No. 5, Shanghai, China.

Abstract

BACKGROUND/AIMS:

Cannabinoids are vasoactive substances that act as key regulators of arterial tone in the blood vessels supplying peripheral tissues and the central nervous system. We therefore investigated the effect of cannabinoids on retinal capillaries and pericytes.

METHODS:

The effects of cannabinoids on capillary diameters were determined using an ex vivo whole-mount rat retinal model. Western blotting, quantitative PCR, and immunohistochemistry were performed to explore the underlying mechanism.

RESULTS:

Endogenous cannabinoid 2-arachidonoylglycerol and anandamide and exogenous cannabinoid (R-(+)-WIN55212-2) dilated the noradrenaline-precontracted capillaries in a concentration-dependent manner (1 µM to 0.1 mM). The extent of vasorelaxation was positively correlated with changes in pericyte width. The effects of R-(+)-WIN55212-2 on vasorelaxation and pericyte width were inhibited by a cannabinoid receptor type-1 (CB1) antagonist, AM251 or rimonabant (SR141716A), the nitric oxide synthase inhibitor l-NAME, and the guanylate cyclase inhibitor ODQ. They were also abolished by the removal of the endothelium, but not by the cannabinoid receptor-2 antagonist SR144528, the endothelial cannabinoid receptor antagonist O-1918, or the cyclooxygenase inhibitor indomethacin.

CONCLUSION:

The exogenous cannabinoid R-(+)-WIN55212-2 promotes the vasorelaxation of pericyte-containing rat retinal capillaries. This effect of R-(+)-WIN55212-2 is dependent on CB1 and the nitric oxide-cyclic guanosine monophosphate pathway, and requires an intact endothelium.

KEYWORDS:

Cannabinoid; Cannabinoid receptor type 1; Pericyte; Retinal capillary; Vasoregulation

PMID:
29059679
DOI:
10.1159/000484193
[Indexed for MEDLINE]
Free full text

Supplemental Content

Full text links

Icon for S. Karger AG, Basel, Switzerland
Loading ...
Support Center