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Protein Cell. 2017 Nov;8(11):823-833. doi: 10.1007/s13238-017-0479-2. Epub 2017 Oct 23.

Recapitulating cortical development with organoid culture in vitro and modeling abnormal spindle-like (ASPM related primary) microcephaly disease.

Li R1, Sun L1, Fang A1,2, Li P1, Wu Q3,4, Wang X5,6,7.

Author information

1
State Key Laboratory of Brain and Cognitive Science, CAS Center for Excellence in Brain Science and Intelligence Technology (Shanghai), Institute of Biophysics, Chinese Academy of Sciences, Beijing, 100101, China.
2
University of Chinese Academy of Sciences, Beijing, 100049, China.
3
State Key Laboratory of Brain and Cognitive Science, CAS Center for Excellence in Brain Science and Intelligence Technology (Shanghai), Institute of Biophysics, Chinese Academy of Sciences, Beijing, 100101, China. qianwu@ibp.ac.cn.
4
University of Chinese Academy of Sciences, Beijing, 100049, China. qianwu@ibp.ac.cn.
5
State Key Laboratory of Brain and Cognitive Science, CAS Center for Excellence in Brain Science and Intelligence Technology (Shanghai), Institute of Biophysics, Chinese Academy of Sciences, Beijing, 100101, China. xiaoqunwang@ibp.ac.cn.
6
University of Chinese Academy of Sciences, Beijing, 100049, China. xiaoqunwang@ibp.ac.cn.
7
Beijing Institute for Brain Disorders, Beijing, 100069, China. xiaoqunwang@ibp.ac.cn.

Abstract

The development of a cerebral organoid culture in vitro offers an opportunity to generate human brain-like organs to investigate mechanisms of human disease that are specific to the neurogenesis of radial glial (RG) and outer radial glial (oRG) cells in the ventricular zone (VZ) and subventricular zone (SVZ) of the developing neocortex. Modeling neuronal progenitors and the organization that produces mature subcortical neuron subtypes during early stages of development is essential for studying human brain developmental diseases. Several previous efforts have shown to grow neural organoid in culture dishes successfully, however we demonstrate a new paradigm that recapitulates neocortical development process with VZ, OSVZ formation and the lamination organization of cortical layer structure. In addition, using patient-specific induced pluripotent stem cells (iPSCs) with dysfunction of the Aspm gene from a primary microcephaly patient, we demonstrate neurogenesis defects result in defective neuronal activity in patient organoids, suggesting a new strategy to study human developmental diseases in central nerve system.

KEYWORDS:

ASPM; cerebral organoid; microcephaly; neocortical development

PMID:
29058117
PMCID:
PMC5676597
DOI:
10.1007/s13238-017-0479-2
[Indexed for MEDLINE]
Free PMC Article

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