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J Coll Physicians Surg Pak. 2017 Oct;27(10):602-605. doi: 2717.

Polymorphisms in the Growth Differentiation Factor 5 (GDF 5) Gene in Knee Osteoarthritis.

Author information

1
Department of Medical Biology, Faculty of Medicine, Bozok University, Yozgat, Turkey.
2
Department of Orthopedics and Traumatology, Faculty of Medicine, Bozok University, Yozgat, Turkey.
3
Department of Physical Medicine and Rehabilitation, Faculty of Medicine, Bozok University, Yozgat, Turkey.
4
Department of Medical Genetic, Faculty of Medicine, Gazi University, Ankara, Turkey.
5
Department of Infectious Diseases and Microbiology, Faculty of Medicine, Bozok University, Yozgat, Turkey.
6
Department of Family Medicine, Faculty of Medicine, Yeditepe University, Istanbul, Turkey.
7
Department of Internal Medicine, Faculty of Medicine, Bozok University, Yozgat, Turkey.

Abstract

OBJECTIVE:

To identify the frequency of the rs143383 SNPin the GDF5 gene, which is located in the 5'-untranslated region of Turkish population with knee osteoarthritis (OA).

STUDY DESIGN:

Acase-control study.

PLACE AND DURATION OF STUDY:

Orthopedics and Traumatology Department, Bozok University Medical Faculty, Yozgat, Turkey, from 2012 to 2014.

METHODOLOGY:

Patients diagnosed with OA(n=94) and patients who did not have joint complaints (n=279) were enrolled in this study. Patients diagnosed with osteoarthritis according to the 1986 American College of Rheumatology osteoarthritis criteria and Kellgren and Lawrence scores were investigated, based on age, gender, and X-ray findings. Blood samples were taken for the identification of GDF5 (rs143383) SNPs by PCR/RFLP, according to a standard protocol.

RESULTS:

This study included 373 patients. The OAgroup (25.2%; n=94) was characterized by specific genotypes: TT (39.4%; n=37); heterozygotes (TC; 45.7%; n=43); and homozygotes (CC; 14.9%; n=14). The control group (74.8%; n=279) was comprised of TT(26.5%; n=74), TC (54.8%; n=153), and CC (18.6%; n=52) genotypes. An analysis of rs143383 SNP of the GDF5 gene polymorphism revealed that the rs143383 TTgenotype had a higher risk for OA(crude OR=1.798, 95% CI=1.010-2.941, p=0.021).

CONCLUSION:

This study demonstrated that there is a correlation of +104T/C polymorphism in the 5'-UTR of GDF5 with knee OAin a Turkish population.

PMID:
29056119
DOI:
2717
[Indexed for MEDLINE]

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