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Gene. 2018 Jan 30;641:78-85. doi: 10.1016/j.gene.2017.10.028. Epub 2017 Oct 17.

A lncRNA-H19 transcript from secondary hair follicle of Liaoning cashmere goat: Identification, regulatory network and expression regulated potentially by its promoter methylation.

Author information

1
College of Animal Science & Veterinary Medicine, Shenyang Agricultural University, Shenyang 110866, PR China.
2
Sichuan Animal Science Academy, Chengdu 610066, PR China.
3
Academy of Animal Husbandry Science of Liaoning Province, Liaoyang 130062, PR China.
4
College of Animal Science & Veterinary Medicine, Shenyang Agricultural University, Shenyang 110866, PR China. Electronic address: baiwenlin55@163.com.

Abstract

The H19 transcript (imprinted maternally expressed transcript) is well-known as long noncoding RNA (lncRNA), and it is thought to be associated with the inductive capacity of dermal papilla cells for hair-follicle reconstruction. In this study, we isolated and characterized a lncRNA-H19 transcript from the secondary hair follicle of Liaoning cashmere goat. Also, we investigated its transcriptional pattern and methylation status of H19 gene in secondary hair follicle of this breed during different stages of hair follicle cycle. Nucleotide composition analysis indicated that guanine (G) and cytosine (C) are the dominant nucleotides in the lncRNA-H19 transcript of Liaoning cashmere goat with the highest frequency distribution (11.25%) of GG nucleotide pair. The regulatory network showed that lncRNA-H19 transcript appears to have remarkably diverse regulatory relationships with its related miRNAs and the potential target genes. In secondary hair follicle, the relative expression of lncRNA-H19 transcript at the anagen phase is significantly higher than that at both telogen and catagen phases suggesting that lncRNA-H19 transcript might play essential roles in the formation and growth of cashmere fiber of goat. Methylation analysis indicated that the methylation of the promoter region of H19 gene most likely participates in its transcriptional suppression in secondary hair follicle of Liaoning cashmere goat.

KEYWORDS:

Expression patterns; Liaoning cashmere goat; Promoter methylation; Regulatory network; Secondary hair follicle; lncRNA-H19

PMID:
29054756
DOI:
10.1016/j.gene.2017.10.028
[Indexed for MEDLINE]

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