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Anal Chim Acta. 2017 Nov 1;992:119-127. doi: 10.1016/j.aca.2017.09.010. Epub 2017 Sep 13.

Simple, high efficiency detection of microcystins and nodularin-R in water by fluorescence polarization immunoassay.

Author information

1
Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Veterinary Medicine, China Agricultural University, Beijing Key Laboratory of Detection Technology for Animal-Derived Food Safety, Beijing Laboratory for Food Quality and Safety, Beijing 100193, People's Republic of China.
2
Institute of Apicultural Research, Chinese Academy of Agricultural Sciences, Bee Product Quality Supervision and Testing Center, Laboratory of Risk Assessment for Quality and Safety of Bee Products, Ministry of Agriculture, Beijing 100093, People's Republic of China.
3
Southern Plains Agricultural Research Center, Agricultural Research Service, U.S. Department of Agriculture, College Station, TX 77845, USA.
4
Faculty of Pharmaceutical Sciences, Laboratory of Food Analysis, Ghent University, Ottergemsesteenweg 460, 9000 Ghent, Belgium.
5
Guangdong Provincial Key Laboratory of Food Quality and Safety, College of Food Science, South China Agricultural University, Guangzhou, 510642, People's Republic of China.
6
State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Synergetic Innovation Center of Food Safety and Nutrition, Jiangnan University, Wuxi, Jiangsu 214122, People's Republic of China.
7
Department of Genetic Toxicology, Shenzhen Center for Disease Control and Prevention, Shenzhen, 518020, People's Republic of China.
8
Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Veterinary Medicine, China Agricultural University, Beijing Key Laboratory of Detection Technology for Animal-Derived Food Safety, Beijing Laboratory for Food Quality and Safety, Beijing 100193, People's Republic of China. Electronic address: wangzhanhui@cau.edu.cn.

Abstract

This manuscript describes the development of a sensitive, fast and easily-performed fluorescence polarization immunoassay (FPIA) for detection of microcystins (MCs) and nodularin-R (NOD) in water. MCs and NOD, the most widespread cyanobacterial toxin are hepatotoxins and tumor promoters, and their acute exposure may result in severe health problems in animals and humans. The fluorescein-based tracers were synthesized, and for the first time preparative high performance liquid chromatography (HPLC) was employed for their purification. Optimal tracers for the analysis were selected by evaluating the immunochemical activity. Under the optimal conditions, the achieved limits of detection (LODs) for MC-LR and NOD were 0.86 and 0.95 μg L-1, respectively, providing a sufficient sensitivity to meet the provisional guideline value recommended by the World Health Organization (WHO). An ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method was developed to confirm the accuracy and precision of the FPIA, and no obvious difference in recovery between these two methods was found. The correlation coefficients (R2) were higher than 0.968. The developed FPIA was easy-to-operate and could be completed within 10 min after simple filtration and adjustment of pH for water samples. The method can be easily extended for screening of other cyanotoxins, representing a versatile strategy for environmental sample analysis.

KEYWORDS:

Fluorescence polarization immunoassay; Homogeneous assay; Microcystins; Nodularin-R; Water analysis

PMID:
29054145
DOI:
10.1016/j.aca.2017.09.010
[Indexed for MEDLINE]

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