Format

Send to

Choose Destination
Anal Chim Acta. 2017 Nov 1;992:105-111. doi: 10.1016/j.aca.2017.09.006. Epub 2017 Sep 13.

Dual-signal model array sensor based on GQDs/AuNPs system for sensitive protein discrimination.

Author information

1
Research Center for Analytical Sciences, Department of Chemistry, Northeastern University, Box 332, Shenyang 110819, China.
2
Research Center for Analytical Sciences, Department of Chemistry, Northeastern University, Box 332, Shenyang 110819, China. Electronic address: chenxuwei@mail.neu.edu.cn.
3
Research Center for Analytical Sciences, Department of Chemistry, Northeastern University, Box 332, Shenyang 110819, China. Electronic address: jianhuajrz@mail.neu.edu.cn.

Abstract

The accurate differentiation and identification of proteins play a vital role in many areas. Herein a novel array sensor is developed for sensitive discrimination of proteins, based on the various optical responses of GQDs/AuNPs system towards different protein species. The simultaneously generated distinct variations of fluorescence and absorbance of GQDs/AuNPs system resulted from the interactions between protein species and sensing units contribute to a dual-signal strategy for protein discrimination. The protein concentration for complete discrimination is low down to 50 nM, and accurate discriminations of protein mixture of different concentrations/molar ratio are achieved. The complementary fluorescence and absorbance response makes this dual-signal model array sensor practicable to sample of complicated matrices, demonstrated by the accurate discrimination of protein species in human urine. Moreover, six strains of microorganisms originated from three different species are also successfully discriminated with 100% accuracy (OD600 = 1.0).

KEYWORDS:

Array sensor; AuNPs; Dual-signal model; GQDs; Protein discrimination

PMID:
29054143
DOI:
10.1016/j.aca.2017.09.006
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center